锌/Co沸石咪唑盐框架上的钯纳米粒子：用于谷胱甘肽双比色和荧光检测的无h2o2氧化酶模拟物

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-01-22 DOI:10.1016/j.snb.2025.137294

Fei-Fei Li, Yu Liu, Wen-Li Jia, Rong-Na Ma, Wei Zhang, Lei Shang, Xiao-Jian Li, Huai-Sheng Wang, Li-Ping Jia

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引用次数: 0

摘要

谷胱甘肽（GSH）是一种重要的抗氧化剂和自由基清除剂，在增强人体免疫系统活性和解毒方面起着至关重要的作用。谷胱甘肽的异常表达与多种疾病有关。本研究合成了一种Pd纳米粒子（PdNPs）修饰Zn/Co分子筛咪唑骨架（ZIF）纳米复合材料（Zn/Co-ZIF@PdNPs）。Zn2 +的掺杂大大提高了Co-ZIF的稳定性。由于Zn/Co-ZIF与PdNPs的协同作用，得到的Zn/Co-ZIF@PdNPs具有优异的类氧化酶活性。Zn/Co-ZIF@PdNPs可以有效催化无色的3,3 ',5,5 ' -四甲基联苯胺（TMB）和非荧光硫胺素（TH）分别氧化为蓝色的3,3 ',5,5 ' -四甲基苯并二亚胺（TMBDI）和荧光硫代色素，而不需要添加氧化剂。在此基础上，利用GSH对TMB和TH氧化的抑制作用，建立了GSH双模比色-荧光检测方法。比色和荧光检测的线性范围为0.3 ~ 12 μM和0.5 ~ 60 μM，检出限分别为0.12 μM和0.015 μM。此外，所开发的生物传感器在人血清和黄瓜样品中显示出良好的可行性。

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Pd nanoparticles on Zn/Co zeolitic imidazolate frameworks: A H2O2-free oxidase mimic for dual colorimetric and fluorescent detection of glutathione

Glutathione (GSH), a key antioxidant and free radical scavenger, plays a crucial role in enhancing the activity of the human immune system and detoxification. The abnormal expression of GSH has been linked to several diseases. In this study, a Pd nanoparticles (PdNPs)-decorated Zn/Co zeolitic imidazole framework (ZIF) nanocomposite (Zn/Co-ZIF@PdNPs) was synthesized. Doping with Zn^2 + greatly enhanced the stability of Co-ZIF. Due to the synergy between Zn/Co-ZIF and PdNPs, the obtained Zn/Co-ZIF@PdNPs exhibited excellent oxidase-like activity. Zn/Co-ZIF@PdNPs could effectively catalyze the oxidation of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) and nonfluorescent thiamine (TH) into blue 3,3′,5,5′-tetramethylbenzodiimine (TMBDI) and fluorescent thiochrome, respectively, without requiring an additional oxidizer. Based on this mechanism, a dual-mode colorimetric–fluorescent detection method for GSH was developed, utilizing the inhibitory effect of GSH on the oxidation of TMB and TH. The colorimetric and fluorescent sensing demonstrated wide linear ranges of 0.3–12 μM and 0.5–60 μM, with detection limits of 0.12 μM and 0.015 μM, respectively. In addition, the developed biosensor exhibited promising feasibility using human serum and cucumber samples.

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.