间隙DNA的序列依赖液晶排序

IF 5.1 Q1 POLYMER SCIENCE

ACS Macro Letters Pub Date : 2025-01-22 DOI:10.1021/acsmacrolett.4c00788

Sineth G. Kodikara, James T. Gleeson, Antal Jakli, Samuel Sprunt, Hamza Balci

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引用次数: 0

摘要

我们利用同步小角度x射线散射和偏振光学显微镜研究了聚腺嘌呤（poly- a）序列对由间隙DNA（由柔性单链桥联的两条双臂）的浓缩溶液形成的液晶（LC）相的类型和稳定性的影响。具有混合序列的样品形成层状（近晶）相，poly-A样品在较低温度（5-35℃）下表现为柱状相，这在GDNA样品中以前没有观察到，并且在较高温度（35-65℃）下表现为异常稳定的近晶- b相。我们提出了一个模型，将这些LC相的形成与poly-A序列的独特特征联系起来，这些特征在各种生物环境中表现出来，包括DNA凝聚和核小体的形成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Sequence-Dependent Liquid Crystalline Ordering of Gapped DNA

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Sequence-Dependent Liquid Crystalline Ordering of Gapped DNA

We investigate the impact of poly adenine (poly-A) sequences on the type and stability of liquid crystalline (LC) phases formed by concentrated solutions of gapped DNA (two duplex arms bridged by a flexible single strand) using synchrotron small-angle X-ray scattering and polarizing optical microscopy. While samples with mixed sequence form layered (smectic) phases, poly-A samples demonstrate a columnar phase at lower temperatures (5–35 °C), not previously observed in GDNA samples, and a smectic-B phase of exceptional stability at higher temperatures (35–65 °C). We present a model that connects the formation of these LC phases with the unique characteristics of poly-A sequences, which manifest in various biological contexts, including DNA condensation and nucleosome formation.

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来源期刊

ACS Macro Letters 化学-

CiteScore

10.40

自引率

3.40%

发文量

209

审稿时长

1 months

期刊介绍： ACS Macro Letters publishes research in all areas of contemporary soft matter science in which macromolecules play a key role, including nanotechnology, self-assembly, supramolecular chemistry, biomaterials, energy generation and storage, and renewable/sustainable materials. Submissions to ACS Macro Letters should justify clearly the rapid disclosure of the key elements of the study. The scope of the journal includes high-impact research of broad interest in all areas of polymer science and engineering, including cross-disciplinary research that interfaces with polymer science. With the launch of ACS Macro Letters, all Communications that were formerly published in Macromolecules and Biomacromolecules will be published as Letters in ACS Macro Letters.