Aspergillus, Penicillium, and Talaromyces (Eurotiales) in Brazilian caves, with the description of four new species.

The study of the Brazilian cave mycobiota has revealed a rich but highly diverse assemblage of fungi, with Aspergillus, Penicillium, and Talaromyces being the most frequently reported genera. The present study investigated the airborne fungi and fungi obtained from the bodies of bats, guano, and the soil/sediment from the caves Urubu (in the Atlantic Forest) and Furna Feia (in the Caatinga dryland forest) in the Northeast region of Brazil. Fungal strains were identified based on morphological features and multilocus phylogenetic analyses of ITS, beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) sequences. A total of 86 isolates were obtained, representing Aspergillus (34), Penicillium (20), Talaromyces (2), and 30 isolates belonging to other genera that will be reported on elsewhere. These isolates were identified as 18 Aspergillus, nine Penicillium, and one Talaromyces species. Eight of the species identified are reported for the first time from a cave environment. Four species showed unique morphological features and phylogenetic relationships, and are newly described. These include two new species of Aspergillus (A. alvaroi sp. nov. and A. guanovespertilionum sp. nov.), one of Penicillium (P. cecavii sp. nov.), and one of Talaromyces (T. potiguarorum sp. nov.). Our study increases the awareness and known richness of the Brazilian and global fungal diversity found in caves. Citation: Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Bezerra JDP, Souza-Motta CM (2024). Aspergillus, Penicillium, and Talaromyces (Eurotiales) in Brazilian caves, with the description of four new species. Fungal Systematics and Evolution 14: 89-107. doi: 10.3114/fuse.2024.14.06.