X Wu, J Wang, S Gao, X Luo, Q Li, D Chen, X Liu, Y Gu, W Li
{"title":"[2020年安徽省绵羊、山羊无原体感染流行情况分析]。","authors":"X Wu, J Wang, S Gao, X Luo, Q Li, D Chen, X Liu, Y Gu, W Li","doi":"10.16250/j.32.1374.2023232","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the prevalence and molecular characteristics of <i>Anaplasma</i> infections in sheep and goats in Anhui Province in 2020, so as to provide insights into ovine anaplasmosis prevention and control.</p><p><strong>Methods: </strong>A total of 355 fresh blood samples were collected from 7 sheep and goat farms in Linquan County of Fuyang City, Lixin County of Bozhou City, Yu'an District of Lu'an City, Wangjiang County of Anqing City, Nanling County of Wuhu City, and Tianchang City and Fengyang County of Chuzhou City in Anhui Province from June to December 2020. <i>A. bovis</i> and <i>A. phagocytophilum</i> 16S ribosomal RNA (<i>16S rRNA</i>) gene, <i>A. ovis</i> major surface protein 4 (<i>MSP4</i>) gene and <i>A. capra</i> citric acid synthase (<i>gltA</i>) gene were amplified using PCR assay in all blood samples, and the prevalence of <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> infections was calculated in sheep and goats. In addition, the positive amplification products were sequenced and subjected to genetic evolutionary analysis.</p><p><strong>Results: </strong>The overall prevalence of <i>Anaplasma</i> infections was 17.5% (62/355) in sheep and goats in Anhui Province, and the prevalence of <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> infections was 2.8% (10/355), 2.5% (9/355), 2.5% (9/355), and 7.0% (25/355), while the prevalence of <i>A. bovis</i> and <i>A. phagocytophilum</i>, <i>A. phagocytophilum</i> and <i>A. ovis</i>, <i>A. phagocytophilum</i> and <i>A. capra</i> and <i>A. bovis</i>, <i>A. phagocytophilum</i> and <i>A. ovis</i> co-infections was 0.8% (3/355), 1.1% (4/355), 0.3% (1/355) and 0.3% (1/355), respectively. No <i>Anaplasma</i> was detected in the sheep and goat farms in Fengyang County, while at least three <i>Anaplasma</i> species were detected in other sheep and goat farms, with co-infections of multiple <i>Anaplasma</i> species identified. The prevalence of <i>Anaplasma</i> infections was 14.7% (24/163) in goats and 19.8% (38/192) in sheep, and the prevalence of <i>Anaplasma</i> infections was 31.0% (31/100) in goats and sheep under 6 months of age, and 12.2% (31/255) in goats and sheep at ages of 6 months and older, respectively. <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> were identified in sheep and goats of different breeds and ages.</p><p><strong>Conclusions: </strong>Multiple <i>Anaplasma</i> species infections were commonly prevalent in goats and sheep in Anhui Province in 2020, notably <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i>, which have zoonotic risks. Improved surveillance and prevention and control of <i>Anaplasma</i> infections are required in sheep and goats in Anhui Province.</p>","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"620-625"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Prevalence of <i>Anaplasma</i> infections in sheep and goats in Anhui Province in 2020].\",\"authors\":\"X Wu, J Wang, S Gao, X Luo, Q Li, D Chen, X Liu, Y Gu, W Li\",\"doi\":\"10.16250/j.32.1374.2023232\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the prevalence and molecular characteristics of <i>Anaplasma</i> infections in sheep and goats in Anhui Province in 2020, so as to provide insights into ovine anaplasmosis prevention and control.</p><p><strong>Methods: </strong>A total of 355 fresh blood samples were collected from 7 sheep and goat farms in Linquan County of Fuyang City, Lixin County of Bozhou City, Yu'an District of Lu'an City, Wangjiang County of Anqing City, Nanling County of Wuhu City, and Tianchang City and Fengyang County of Chuzhou City in Anhui Province from June to December 2020. <i>A. bovis</i> and <i>A. phagocytophilum</i> 16S ribosomal RNA (<i>16S rRNA</i>) gene, <i>A. ovis</i> major surface protein 4 (<i>MSP4</i>) gene and <i>A. capra</i> citric acid synthase (<i>gltA</i>) gene were amplified using PCR assay in all blood samples, and the prevalence of <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> infections was calculated in sheep and goats. In addition, the positive amplification products were sequenced and subjected to genetic evolutionary analysis.</p><p><strong>Results: </strong>The overall prevalence of <i>Anaplasma</i> infections was 17.5% (62/355) in sheep and goats in Anhui Province, and the prevalence of <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> infections was 2.8% (10/355), 2.5% (9/355), 2.5% (9/355), and 7.0% (25/355), while the prevalence of <i>A. bovis</i> and <i>A. phagocytophilum</i>, <i>A. phagocytophilum</i> and <i>A. ovis</i>, <i>A. phagocytophilum</i> and <i>A. capra</i> and <i>A. bovis</i>, <i>A. phagocytophilum</i> and <i>A. ovis</i> co-infections was 0.8% (3/355), 1.1% (4/355), 0.3% (1/355) and 0.3% (1/355), respectively. No <i>Anaplasma</i> was detected in the sheep and goat farms in Fengyang County, while at least three <i>Anaplasma</i> species were detected in other sheep and goat farms, with co-infections of multiple <i>Anaplasma</i> species identified. The prevalence of <i>Anaplasma</i> infections was 14.7% (24/163) in goats and 19.8% (38/192) in sheep, and the prevalence of <i>Anaplasma</i> infections was 31.0% (31/100) in goats and sheep under 6 months of age, and 12.2% (31/255) in goats and sheep at ages of 6 months and older, respectively. <i>A. bovis</i>, <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i> were identified in sheep and goats of different breeds and ages.</p><p><strong>Conclusions: </strong>Multiple <i>Anaplasma</i> species infections were commonly prevalent in goats and sheep in Anhui Province in 2020, notably <i>A. phagocytophilum</i>, <i>A. ovis</i> and <i>A. capra</i>, which have zoonotic risks. Improved surveillance and prevention and control of <i>Anaplasma</i> infections are required in sheep and goats in Anhui Province.</p>\",\"PeriodicalId\":38874,\"journal\":{\"name\":\"中国血吸虫病防治杂志\",\"volume\":\"36 6\",\"pages\":\"620-625\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-07-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国血吸虫病防治杂志\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.16250/j.32.1374.2023232\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国血吸虫病防治杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.16250/j.32.1374.2023232","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
[Prevalence of Anaplasma infections in sheep and goats in Anhui Province in 2020].
Objective: To investigate the prevalence and molecular characteristics of Anaplasma infections in sheep and goats in Anhui Province in 2020, so as to provide insights into ovine anaplasmosis prevention and control.
Methods: A total of 355 fresh blood samples were collected from 7 sheep and goat farms in Linquan County of Fuyang City, Lixin County of Bozhou City, Yu'an District of Lu'an City, Wangjiang County of Anqing City, Nanling County of Wuhu City, and Tianchang City and Fengyang County of Chuzhou City in Anhui Province from June to December 2020. A. bovis and A. phagocytophilum 16S ribosomal RNA (16S rRNA) gene, A. ovis major surface protein 4 (MSP4) gene and A. capra citric acid synthase (gltA) gene were amplified using PCR assay in all blood samples, and the prevalence of A. bovis, A. phagocytophilum, A. ovis and A. capra infections was calculated in sheep and goats. In addition, the positive amplification products were sequenced and subjected to genetic evolutionary analysis.
Results: The overall prevalence of Anaplasma infections was 17.5% (62/355) in sheep and goats in Anhui Province, and the prevalence of A. bovis, A. phagocytophilum, A. ovis and A. capra infections was 2.8% (10/355), 2.5% (9/355), 2.5% (9/355), and 7.0% (25/355), while the prevalence of A. bovis and A. phagocytophilum, A. phagocytophilum and A. ovis, A. phagocytophilum and A. capra and A. bovis, A. phagocytophilum and A. ovis co-infections was 0.8% (3/355), 1.1% (4/355), 0.3% (1/355) and 0.3% (1/355), respectively. No Anaplasma was detected in the sheep and goat farms in Fengyang County, while at least three Anaplasma species were detected in other sheep and goat farms, with co-infections of multiple Anaplasma species identified. The prevalence of Anaplasma infections was 14.7% (24/163) in goats and 19.8% (38/192) in sheep, and the prevalence of Anaplasma infections was 31.0% (31/100) in goats and sheep under 6 months of age, and 12.2% (31/255) in goats and sheep at ages of 6 months and older, respectively. A. bovis, A. phagocytophilum, A. ovis and A. capra were identified in sheep and goats of different breeds and ages.
Conclusions: Multiple Anaplasma species infections were commonly prevalent in goats and sheep in Anhui Province in 2020, notably A. phagocytophilum, A. ovis and A. capra, which have zoonotic risks. Improved surveillance and prevention and control of Anaplasma infections are required in sheep and goats in Anhui Province.
期刊介绍：
Chinese Journal of Schistosomiasis Control (ISSN: 1005-6661, CN: 32-1374/R), founded in 1989, is a technical and scientific journal under the supervision of Jiangsu Provincial Health Commission and organised by Jiangsu Institute of Schistosomiasis Control. It is a scientific and technical journal under the supervision of Jiangsu Provincial Health Commission and sponsored by Jiangsu Institute of Schistosomiasis Prevention and Control. The journal carries out the policy of prevention-oriented, control-oriented, nationwide and grassroots, adheres to the tenet of scientific research service for the prevention and treatment of schistosomiasis and other parasitic diseases, and mainly publishes academic papers reflecting the latest achievements and dynamics of prevention and treatment of schistosomiasis and other parasitic diseases, scientific research and management, etc. The main columns are Guest Contributions, Experts‘ Commentary, Experts’ Perspectives, Experts' Forums, Theses, Prevention and Treatment Research, Experimental Research, The main columns include Guest Contributions, Expert Commentaries, Expert Perspectives, Expert Forums, Treatises, Prevention and Control Studies, Experimental Studies, Clinical Studies, Prevention and Control Experiences, Prevention and Control Management, Reviews, Case Reports, and Information, etc. The journal is a useful reference material for the professional and technical personnel of schistosomiasis and parasitic disease prevention and control research, management workers, and teachers and students of medical schools.
The journal is now included in important domestic databases, such as Chinese Core List (8th edition), China Science Citation Database (Core Edition), China Science and Technology Core Journals (Statistical Source Journals), and is also included in MEDLINE/PubMed, Scopus, EBSCO, Chemical Abstract, Embase, Zoological Record, JSTChina, Ulrichsweb, Western Pacific Region Index Medicus, CABI and other international authoritative databases.
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