对成年斑马鱼进行离体视网膜电成像的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-03-21 Epub Date: 2025-01-16 DOI:10.1016/j.xpro.2024.103565

Samantha L Simpson, Ross F Collery

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引用次数: 0

摘要

离体视网膜电图（ERG）提供了对视网膜细胞的健康和功能、光导的完整性和视觉周期的深入了解，并允许将药物直接应用于视网膜组织。在这里，我们提出了一种对成年斑马鱼进行体外ERG的方案。我们描述斑马鱼组织解剖，安装组织，组装和连接磁带的步骤。然后，我们详细介绍了运行诊断软件以及处理和分析结果数据的过程。

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Protocol to perform ex vivo electroretinography on adult zebrafish.

Ex vivo electroretinography (ERG) provides insight into the health and functionality of retinal cells, the integrity of phototransduction, and the visual cycle and allows for the direct application of pharmaceuticals to the retinal tissues. Here, we present a protocol for performing ex vivo ERG on adult zebrafish. We describe steps for zebrafish tissue dissection, mounting tissues, and assembling and connecting cassettes. We then detail procedures for running the Diagnosys software and processing and analyzing the resulting data.

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