冷暴露和CL316,243注射诱导小鼠白色脂肪组织中米色脂肪细胞的方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-03-21 Epub Date: 2025-01-15 DOI:10.1016/j.xpro.2024.103337

Yalan Wu, Chenguang Wang, Xiao Yu Tian

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引用次数: 0

摘要

白色脂肪组织（WAT）在对抗肥胖方面具有重要的治疗潜力。在这里，我们提出了一种使用冷暴露和CL316,243处理诱导小鼠米色WAT的方案。我们描述了腹膜内注射和皮下WAT （sWAT）分离、解剖和固定的步骤。然后我们详细介绍了组织学，全挂载免疫荧光（IF）染色和提取RNA和蛋白质的程序。该方案可用于后续分析，以探索实验环境中米色WAT诱导的机制，特别是血管生成的评估。有关本协议使用和执行的完整细节，请参考Wang等人1。

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Protocol for inducing beige adipocytes in white adipose tissue of mouse using cold exposure and CL316,243 injection.

White adipose tissue (WAT) beiging holds significant therapeutic potential for combating obesity. Here, we present a protocol for inducing beige WAT in mice using both cold exposure and CL316,243 treatment. We describe steps for intraperitoneal injection, and subcutaneous WAT (sWAT) isolation, dissection, and fixation. We then detail procedures for histology, whole-mount immunofluorescence (IF) staining, and extracting RNA and protein. This protocol can be used for subsequent analysis to explore the mechanisms governing beige WAT induction in experimental settings, particularly the evaluation of angiogenesis. For complete details on the use and execution of this protocol, please refer to Wang et al.¹.

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