南欧灰狼（Canis lupus）嗜血支原体的发生及分子鉴定。

IF 2.4 2区农林科学 Q3 MICROBIOLOGY

Veterinary microbiology Pub Date : 2025-01-12 DOI:10.1016/j.vetmic.2025.110390

Susana Remesar , David Cano-Terriza , Patrocinio Morrondo , Álvaro Oleaga , Barbara Moroni , Nuno Santos , Serena Robetto , Lisa Guardone , Pablo Díaz , Saúl Jiménez-Ruiz , Joana Ferreira-e-Silva , Moisés Gonzálvez , Ignacio García-Bocanegra

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引用次数: 0

摘要

尽管野生和家养食肉动物都有一些嗜血性支原体，但关于这种病原体在灰狼（Canis lupus）种群中传播的信息仍然非常有限。因此，进行了一项地理基础广泛的调查，以确定支原体在南欧三个不同狼种群中的发生和多样性。在2001年至2023年间，从西班牙（n = 129）、意大利（n = 113）和葡萄牙（n = 43）的285只灰狼身上采集脾脏样本。以16S rRNA基因为靶点，采用两种PCR方法检测嗜血性支原体的存在；此外，分析16S-23S rRNA基因间间隔，进一步鉴定阳性样品。通过广义线性模型分析，还评估了采样国家、性别和动物年龄对支原体流行率的影响。阳性率为13.3 %(38/285)，其中西班牙（20.9 %）明显高于意大利（8.0 %）和葡萄牙（4.7 %）。鉴定出血支原体（10.5% %）和血疟原虫（2.1% %）；此外，还检出未培养的支原体（0.7 %）。我们的结果证实了潜在的人畜共患支原体在南欧狼种群中的传播。据我们所知，这是首次在意大利和葡萄牙的狼中发现血疟原虫。此外，以前在狗身上发现的支原体sp.首次在狼身上发现。需要进一步的研究来充分表征嗜血性支原体的分子特征，这将为其生态流行病学研究奠定基础。

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Occurrence and molecular identification of haemotropic Mycoplasma species in grey wolves (Canis lupus) from southern Europe

Although wild and domestic carnivores share some haemotropic Mycoplasma species, information about the circulation of this pathogen in grey wolves (Canis lupus) populations is still very limited. Thus, a geographically broad-based investigation was performed for determining the occurrence and diversity of Mycoplasma spp. in three different wolf populations from southern Europe. Between 2001 and 2023, spleen samples from 285 grey wolves from Spain (n = 129), Italy (n = 113), and Portugal (n = 43) were collected. The presence of haemotropic Mycoplasma was assessed targeting the 16S rRNA gene using two PCR assays in parallel; in addition, the 16S–23S rRNA intergenic spacer was analysed for further identification of the positive samples. The influence of the sampling country, sex, and age of the animals on the prevalence of Mycoplasma spp. was also assessed by a generalized linear model analysis. The percentage of positive wolves was 13.3 % (38/285), and the occurrence was significantly higher in Spain (20.9 %) than in Italy (8.0 %) and Portugal (4.7 %). Mycoplasma haemocanis (10.5 %) and Candidatus M. haematoparvum (2.1 %), were identified; in addition, an uncultured Mycoplasma sp. was also detected (0.7 %). Our results confirm the circulation of potentially zoonotic Mycoplasma in wolf populations from southern Europe. To our knowledge, this is the first report of Ca. M. haematoparvum in wolves from Italy and Portugal. In addition, a Mycoplasma sp., previously found in dogs, has been detected for the first time in wolves. Further studies are needed to fully molecularly characterise haemotropic Mycoplasma spp., which will serve as a basis for the study of its ecoepidemiology.

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来源期刊

Veterinary microbiology 农林科学-兽医学

CiteScore

5.90

自引率

6.10%

发文量

221

审稿时长

52 days

期刊介绍： Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal. Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge. Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.