Rafik El-Mernissi, Naoual El Menyiy, Amira Metouekel, Aziz Zouhri, Yahya El-Mernissi, Farhan Siddique, Sumaira Nadeem, Hassan Amhamdi, Oualid Abboussi, Abdulaziz Abdullah Alsahli, Mohammed Bourhia, Musaab Dauelbait, Gamal A Shazly, Lhoussain Hajji
{"title":"大麻种子中酚类化合物的表征和抗糖尿病潜能的评价：体内、体外和计算机研究。","authors":"Rafik El-Mernissi, Naoual El Menyiy, Amira Metouekel, Aziz Zouhri, Yahya El-Mernissi, Farhan Siddique, Sumaira Nadeem, Hassan Amhamdi, Oualid Abboussi, Abdulaziz Abdullah Alsahli, Mohammed Bourhia, Musaab Dauelbait, Gamal A Shazly, Lhoussain Hajji","doi":"10.1515/biol-2022-1024","DOIUrl":null,"url":null,"abstract":"<p><p>Moroccan <i>Cannabis sativa</i> L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. <i>In vitro</i>, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). <i>In vivo</i> studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of <i>C. sativa</i> seeds as a natural source of bioactive compounds for diabetes management.</p>","PeriodicalId":19605,"journal":{"name":"Open Life Sciences","volume":"19 1","pages":"20221024"},"PeriodicalIF":1.7000,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736388/pdf/","citationCount":"0","resultStr":"{\"title\":\"Characterization of phenolic compounds and evaluation of anti-diabetic potential in <i>Cannabis sativa</i> L. seeds: <i>In vivo, in vitro,</i> and <i>in silico</i> studies.\",\"authors\":\"Rafik El-Mernissi, Naoual El Menyiy, Amira Metouekel, Aziz Zouhri, Yahya El-Mernissi, Farhan Siddique, Sumaira Nadeem, Hassan Amhamdi, Oualid Abboussi, Abdulaziz Abdullah Alsahli, Mohammed Bourhia, Musaab Dauelbait, Gamal A Shazly, Lhoussain Hajji\",\"doi\":\"10.1515/biol-2022-1024\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Moroccan <i>Cannabis sativa</i> L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. <i>In vitro</i>, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). <i>In vivo</i> studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of <i>C. sativa</i> seeds as a natural source of bioactive compounds for diabetes management.</p>\",\"PeriodicalId\":19605,\"journal\":{\"name\":\"Open Life Sciences\",\"volume\":\"19 1\",\"pages\":\"20221024\"},\"PeriodicalIF\":1.7000,\"publicationDate\":\"2024-12-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11736388/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Open Life Sciences\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1515/biol-2022-1024\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Open Life Sciences","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1515/biol-2022-1024","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}
Characterization of phenolic compounds and evaluation of anti-diabetic potential in Cannabis sativa L. seeds: In vivo, in vitro, and in silico studies.
Moroccan Cannabis sativa L. seeds were investigated for their phenolic profile and antidiabetic potential. Ultra-high-performance liquid chromatography with diode array detection and electrospray ionization mass spectrometry analysis revealed a rich phenolic composition, including benzoic acid, cannabisin B, genistein, and epicatechin. In vitro, the seed extract exhibited potent α-amylase inhibitory activity (half-maximal inhibitory concentration = 25.02 ± 4.03 μg/mL). In vivo studies in diabetic rats demonstrated significant hypoglycemic, hypolipidemic, hepatoprotective, and nephroprotective effects. Molecular docking studies further supported these findings, revealing strong interactions between identified phenolic and the α-amylase enzyme. These results highlight the potential of C. sativa seeds as a natural source of bioactive compounds for diabetes management.
期刊介绍：
Open Life Sciences (previously Central European Journal of Biology) is a fast growing peer-reviewed journal, devoted to scholarly research in all areas of life sciences, such as molecular biology, plant science, biotechnology, cell biology, biochemistry, biophysics, microbiology and virology, ecology, differentiation and development, genetics and many others. Open Life Sciences assures top quality of published data through critical peer review and editorial involvement throughout the whole publication process. Thanks to the Open Access model of publishing, it also offers unrestricted access to published articles for all users.
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