膜蛋白在其原生膜环境中的捕获。

IF 36.1 1区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Nature Methods Pub Date : 2025-01-20 DOI:10.1038/s41592-024-02518-w

引用次数: 0

摘要

这项工作提出了优化的实验方案，用于直接从天然膜中提取2000种独特的膜蛋白到天然纳米圆盘中。结合正交结构、生化或生物物理方法，这些天然纳米盘使膜蛋白能够直接在其天然膜环境中进行研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Capture of membrane proteins in their native membrane milieu

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Capture of membrane proteins in their native membrane milieu

This work presents optimized experimental protocols for the extraction of >2,000 unique membrane proteins directly from their native membranes into native nanodiscs. Combined with orthogonal structural, biochemical or biophysical approaches, these native nanodiscs enable membrane proteins to be studied directly in their native membrane context.

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来源期刊

Nature Methods 生物-生化研究方法

CiteScore

58.70

自引率

1.70%

发文量

326

审稿时长

1 months

期刊介绍： Nature Methods is a monthly journal that focuses on publishing innovative methods and substantial enhancements to fundamental life sciences research techniques. Geared towards a diverse, interdisciplinary readership of researchers in academia and industry engaged in laboratory work, the journal offers new tools for research and emphasizes the immediate practical significance of the featured work. It publishes primary research papers and reviews recent technical and methodological advancements, with a particular interest in primary methods papers relevant to the biological and biomedical sciences. This includes methods rooted in chemistry with practical applications for studying biological problems.