Nycolas Octavio Ribeiro Carvalho, Raquel Cuba Gaspar, Suellen Gonçalves Lima, Dayane da Silva Zanini, Nássarah Jabur Lot Rodrigues, Victor Yunes Guimarães, Aristeu Vieira da Silva, Sheila Canevese Rahal, Anna Luisa Pizzaia Henrique, Felipe Fornazari, Helio Langoni
{"title":"间接荧光抗体法与改良凝集法检测新热带灵长类弓形虫IgG抗体的比较","authors":"Nycolas Octavio Ribeiro Carvalho, Raquel Cuba Gaspar, Suellen Gonçalves Lima, Dayane da Silva Zanini, Nássarah Jabur Lot Rodrigues, Victor Yunes Guimarães, Aristeu Vieira da Silva, Sheila Canevese Rahal, Anna Luisa Pizzaia Henrique, Felipe Fornazari, Helio Langoni","doi":"10.1111/jmp.70002","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p><i>Toxoplasma gondii</i> is a zoonotic parasite with a great impact on the health of Neotropical primates (NP). The aim of this study was to compare the agreement between the immunofluorescent antibody test (IFAT) and the modified agglutination test (MAT) to detect IgG anti-<i>T</i>. <i>gondii</i> antibodies in NP.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Convenience serum samples of 77 animals were tested by IFAT and MAT according to standard protocols, and the agreement between results was analyzed using McNemar's <i>χ</i><sup>2</sup> test.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The samples included 30 red-handed howlers (<i>Alouatta belzebul</i>), 24 brown howler monkeys (<i>Alouatta guariba</i>), 22 capuchin monkeys (<i>Sapajus nigritus</i>), and one wedge-capped capuchin (<i>Cebus olivaceous</i>). Overall prevalence was 25.9% (<i>n</i> = 20), with three animals positive by IFAT and 20 positives by MAT. The agreement between tests was low (Kappa = 0.21).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>The results indicated that IFAT and MAT are not comparable to assess qualitatively anti-<i>T</i>. <i>gondii</i> IgG serum antibodies in the studied species.</p>\n </section>\n </div>","PeriodicalId":16439,"journal":{"name":"Journal of Medical Primatology","volume":"54 1","pages":""},"PeriodicalIF":0.8000,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison Between Indirect Fluorescent Antibody Test and Modified Agglutination Test for Detecting Anti-Toxoplasma gondii IgG Antibodies in Neotropical Primates\",\"authors\":\"Nycolas Octavio Ribeiro Carvalho, Raquel Cuba Gaspar, Suellen Gonçalves Lima, Dayane da Silva Zanini, Nássarah Jabur Lot Rodrigues, Victor Yunes Guimarães, Aristeu Vieira da Silva, Sheila Canevese Rahal, Anna Luisa Pizzaia Henrique, Felipe Fornazari, Helio Langoni\",\"doi\":\"10.1111/jmp.70002\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p><i>Toxoplasma gondii</i> is a zoonotic parasite with a great impact on the health of Neotropical primates (NP). The aim of this study was to compare the agreement between the immunofluorescent antibody test (IFAT) and the modified agglutination test (MAT) to detect IgG anti-<i>T</i>. <i>gondii</i> antibodies in NP.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Convenience serum samples of 77 animals were tested by IFAT and MAT according to standard protocols, and the agreement between results was analyzed using McNemar's <i>χ</i><sup>2</sup> test.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>The samples included 30 red-handed howlers (<i>Alouatta belzebul</i>), 24 brown howler monkeys (<i>Alouatta guariba</i>), 22 capuchin monkeys (<i>Sapajus nigritus</i>), and one wedge-capped capuchin (<i>Cebus olivaceous</i>). Overall prevalence was 25.9% (<i>n</i> = 20), with three animals positive by IFAT and 20 positives by MAT. The agreement between tests was low (Kappa = 0.21).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>The results indicated that IFAT and MAT are not comparable to assess qualitatively anti-<i>T</i>. <i>gondii</i> IgG serum antibodies in the studied species.</p>\\n </section>\\n </div>\",\"PeriodicalId\":16439,\"journal\":{\"name\":\"Journal of Medical Primatology\",\"volume\":\"54 1\",\"pages\":\"\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-01-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Medical Primatology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jmp.70002\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medical Primatology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jmp.70002","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Comparison Between Indirect Fluorescent Antibody Test and Modified Agglutination Test for Detecting Anti-Toxoplasma gondii IgG Antibodies in Neotropical Primates
Background
Toxoplasma gondii is a zoonotic parasite with a great impact on the health of Neotropical primates (NP). The aim of this study was to compare the agreement between the immunofluorescent antibody test (IFAT) and the modified agglutination test (MAT) to detect IgG anti-T. gondii antibodies in NP.
Methods
Convenience serum samples of 77 animals were tested by IFAT and MAT according to standard protocols, and the agreement between results was analyzed using McNemar's χ2 test.
Results
The samples included 30 red-handed howlers (Alouatta belzebul), 24 brown howler monkeys (Alouatta guariba), 22 capuchin monkeys (Sapajus nigritus), and one wedge-capped capuchin (Cebus olivaceous). Overall prevalence was 25.9% (n = 20), with three animals positive by IFAT and 20 positives by MAT. The agreement between tests was low (Kappa = 0.21).
Conclusion
The results indicated that IFAT and MAT are not comparable to assess qualitatively anti-T. gondii IgG serum antibodies in the studied species.
期刊介绍：
The Journal of Medical Primatology publishes research on non-human primates as models to study, prevent, and/or treat human diseases; subjects include veterinary medicine; morphology, physiology, reproductive biology, central nervous system, and cardiovascular diseases; husbandry, handling, experimental methodology, and management of non-human primate colonies and laboratories; non-human primate wildlife management; and behaviour and sociology as related to medical conditions and captive non-human primate needs.
Published material includes: Original Manuscripts - research results; Case Reports - scientific documentation of a single clinical study; Short Papers - case histories, methodologies, and techniques of particular interest; Letters to the Editor - opinions, controversies and sporadic scientific observations; Perspectives – opinion piece about existing research on a particular topic; Minireviews – a concise review of existing literature; Book Reviews by invitation; Special Issues containing selected papers from specialized meetings; and Editorials and memoriams authored by the Editor-in-Chief.
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