PLL-g-HPA水凝胶负载人脐带间充质干细胞通过调节炎症反应促进大鼠烧伤创面愈合。

IF 4.2 2区 医学 Q2 IMMUNOLOGY
Journal of Inflammation Research Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI:10.2147/JIR.S492465
Linqiang Tian, Zhaodong Wang, Tingting Lei, Lili Feng, Yanyan Li, Kunxi Wang, Yue Zhang, Chengshu Zhang, Jie Liu, Hongxia Xing, Wenjing Ren
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引用次数: 0

摘要

目的:严重烧伤创面的严重感染/复杂的修复过程和不调节的炎症反应仍然是一个重大的临床挑战。人脐带间充质干细胞(hUC-MSCs)具有多向分化潜能,在适当条件下可修复多种损伤。聚(l -赖氨酸)-接枝-4-羟基苯基乙酸(PLL-g-HPA)水凝胶是一种酶促生物可降解的水凝胶,具有良好的吸水性、生物相容性和抗菌性能。因此,本研究旨在评价hUC-MSCs联合PLL-g-HPA水凝胶对大鼠全层烧伤模型的治疗效果。方法:制备PLL-g-HPA水凝胶,并采用扫描电镜(SEM)、傅里叶变换红外光谱(FTIR)、氢-1核磁共振(H-NMR)对其进行表征。采用CCK-8法和活/死定量法检测其对人包皮成纤维细胞(HFF)的细胞毒性,采用菌落形成单元法检测其对大肠杆菌和金黄色葡萄球菌的抑菌活性。建立12只SD大鼠全层烧伤损伤模型,通过愈合时间/组织学/炎症因子表达水平检测PLL-g-HPA水凝胶联合hUC-MSCs的治疗效果。结果:SEM, FTIR和HFF分析结果表明,成功合成了PLL-g-HPA水凝胶。这些水凝胶在最低浓度下表现出较低的细胞毒性,同时保持良好的保湿性和抗菌性能。与对照组相比,在大鼠模型中,PLL-g-HPA水凝胶联合hUC-MSCs显著增强了伤口愈合,调节了炎症反应,促进了血管生成和再上皮化。结论:PLL-g-HPA水凝胶联合hUC-MSCs是治疗烧伤创面的一种很有前景的治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
PLL-g-HPA Hydrogel Loaded Human Umbilical Cord Mesenchymal Stem Cells Promote Burn Wound Healing in Rat Model by Regulating Inflammation Response.

Purpose: Treatment of severe burn wound injury remains a significant clinical challenge as serious infections/complex repair process and irregulating inflammation response. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have a multidirectional differentiation potential and could repair multiple injuries under appropriate conditions. Poly(L-lysine)-graft-4-hydroxyphenylacetic acid (PLL-g-HPA) hydrogel is an enzyme-promoted biodegradable in hydrogel with good water absorption, biocompatibility and anti-bacterial properties. Therefore, the aim of this study was to evaluate the therapeutic effect of hUC-MSCs combined with PLL-g-HPA hydrogel on full thickness burn injury in rat model.

Methods: The PLL-g-HPA hydrogel was developed and characterized by Scanning Electron Microscopy (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), Hydrogen-1 nuclear magnetic resonance (H-NMR). The cytotoxicity to human foreskin fibroblasts (HFF) were assessed by CCK-8 assay and live/dead quantification and antibacterial activity against Escherichia coli and Staphylococcus aureus was also detected by colony forming unit. A full-thickness burn wound injury model in 12 SD rats was established, and the therapeutic effect of PLL-g-HPA hydrogel combined with hUC-MSCs was detected by healing time/Histology/inflammation factor expression level.

Results: The findings from SEM, FTIR, and HFF analyses demonstrated the successful synthesis of PLL-g-HPA hydrogels. These hydrogels exhibited low cytotoxicity at minimal concentrations while maintaining excellent moisture retention and antibacterial properties. Compared to the control group, treatment with PLL-g-HPA hydrogel in conjunction with hUC-MSCs significantly enhanced wound healing, modulated inflammatory responses, and promoted angiogenesis as well as re-epithelialization in rat models.

Conclusion: The PLL-g-HPA hydrogel in conjunction with hUC-MSCs represents a promising therapeutic approach for the management of burn wounds.

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来源期刊
Journal of Inflammation Research
Journal of Inflammation Research Immunology and Microbiology-Immunology
CiteScore
6.10
自引率
2.20%
发文量
658
审稿时长
16 weeks
期刊介绍: An international, peer-reviewed, open access, online journal that welcomes laboratory and clinical findings on the molecular basis, cell biology and pharmacology of inflammation.
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