{"title":"揭示酒渣鼻的分子机制：来自转录组学和体外实验的见解。","authors":"Luzhu Chen, Juan Wang","doi":"10.1111/jocd.16753","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Rosacea is a prevalent inflammatory skin condition, but its molecular mechanisms and treatment responses remain poorly understood.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study aims to investigate the molecular mechanisms underlying rosacea and explore drug response through transcriptomic analysis and in vitro experiments.</p>\n </section>\n \n <section>\n \n <h3> Patients/Methods</h3>\n \n <p>We performed high-throughput RNA sequencing to analyze gene expression patterns in rosacea patients. In vitro experiments, including RT-qPCR, Western blot, ELISA, scratch, and Transwell assays, were used to evaluate gene and protein expression and cell behavior in HaCaT cells under simulated rosacea conditions.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Transcriptomic analysis revealed significantly elevated expression of inflammatory-related genes in rosacea patients. In vitro, HaCaT cells exhibited enhanced proliferation and migration abilities, accompanied by increased expression of pro-inflammatory genes and proteins. Specifically, Toll-like receptor 2 (TLR2) and S100A9 proteins were upregulated, potentially promoting these processes.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>Our study elucidates the molecular mechanisms of rosacea, highlighting the role of inflammatory pathways and altered cell behavior in the disease. TLR2 and S100A9 may contribute to disease progression, offering potential targets for future therapeutic strategies.</p>\n </section>\n </div>","PeriodicalId":15546,"journal":{"name":"Journal of Cosmetic Dermatology","volume":"24 1","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739675/pdf/","citationCount":"0","resultStr":"{\"title\":\"Unveiling the Molecular Mechanisms of Rosacea: Insights From Transcriptomics and In Vitro Experiments\",\"authors\":\"Luzhu Chen, Juan Wang\",\"doi\":\"10.1111/jocd.16753\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Rosacea is a prevalent inflammatory skin condition, but its molecular mechanisms and treatment responses remain poorly understood.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Aims</h3>\\n \\n <p>This study aims to investigate the molecular mechanisms underlying rosacea and explore drug response through transcriptomic analysis and in vitro experiments.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Patients/Methods</h3>\\n \\n <p>We performed high-throughput RNA sequencing to analyze gene expression patterns in rosacea patients. In vitro experiments, including RT-qPCR, Western blot, ELISA, scratch, and Transwell assays, were used to evaluate gene and protein expression and cell behavior in HaCaT cells under simulated rosacea conditions.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Transcriptomic analysis revealed significantly elevated expression of inflammatory-related genes in rosacea patients. In vitro, HaCaT cells exhibited enhanced proliferation and migration abilities, accompanied by increased expression of pro-inflammatory genes and proteins. Specifically, Toll-like receptor 2 (TLR2) and S100A9 proteins were upregulated, potentially promoting these processes.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>Our study elucidates the molecular mechanisms of rosacea, highlighting the role of inflammatory pathways and altered cell behavior in the disease. TLR2 and S100A9 may contribute to disease progression, offering potential targets for future therapeutic strategies.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15546,\"journal\":{\"name\":\"Journal of Cosmetic Dermatology\",\"volume\":\"24 1\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-01-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11739675/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Cosmetic Dermatology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jocd.16753\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"DERMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cosmetic Dermatology","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jocd.16753","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}
Unveiling the Molecular Mechanisms of Rosacea: Insights From Transcriptomics and In Vitro Experiments
Background
Rosacea is a prevalent inflammatory skin condition, but its molecular mechanisms and treatment responses remain poorly understood.
Aims
This study aims to investigate the molecular mechanisms underlying rosacea and explore drug response through transcriptomic analysis and in vitro experiments.
Patients/Methods
We performed high-throughput RNA sequencing to analyze gene expression patterns in rosacea patients. In vitro experiments, including RT-qPCR, Western blot, ELISA, scratch, and Transwell assays, were used to evaluate gene and protein expression and cell behavior in HaCaT cells under simulated rosacea conditions.
Results
Transcriptomic analysis revealed significantly elevated expression of inflammatory-related genes in rosacea patients. In vitro, HaCaT cells exhibited enhanced proliferation and migration abilities, accompanied by increased expression of pro-inflammatory genes and proteins. Specifically, Toll-like receptor 2 (TLR2) and S100A9 proteins were upregulated, potentially promoting these processes.
Conclusions
Our study elucidates the molecular mechanisms of rosacea, highlighting the role of inflammatory pathways and altered cell behavior in the disease. TLR2 and S100A9 may contribute to disease progression, offering potential targets for future therapeutic strategies.
期刊介绍：
The Journal of Cosmetic Dermatology publishes high quality, peer-reviewed articles on all aspects of cosmetic dermatology with the aim to foster the highest standards of patient care in cosmetic dermatology. Published quarterly, the Journal of Cosmetic Dermatology facilitates continuing professional development and provides a forum for the exchange of scientific research and innovative techniques.
The scope of coverage includes, but will not be limited to: healthy skin; skin maintenance; ageing skin; photodamage and photoprotection; rejuvenation; biochemistry, endocrinology and neuroimmunology of healthy skin; imaging; skin measurement; quality of life; skin types; sensitive skin; rosacea and acne; sebum; sweat; fat; phlebology; hair conservation, restoration and removal; nails and nail surgery; pigment; psychological and medicolegal issues; retinoids; cosmetic chemistry; dermopharmacy; cosmeceuticals; toiletries; striae; cellulite; cosmetic dermatological surgery; blepharoplasty; liposuction; surgical complications; botulinum; fillers, peels and dermabrasion; local and tumescent anaesthesia; electrosurgery; lasers, including laser physics, laser research and safety, vascular lasers, pigment lasers, hair removal lasers, tattoo removal lasers, resurfacing lasers, dermal remodelling lasers and laser complications.
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