Jianwei Qin, Yang Liu, Mingming Cao, Yue Zhang, Guangdong Bai, Baoming Shi
{"title":"枯草芽孢杆菌MZ-01可减轻ETEC K88引起的腹泻，减少炎症，促进肠道健康。","authors":"Jianwei Qin, Yang Liu, Mingming Cao, Yue Zhang, Guangdong Bai, Baoming Shi","doi":"10.1093/jambio/lxaf018","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>The purpose of this study was to investigate the effects of Bacillus subtilis supplementation on the health of weaned piglets and whether Bacillus subtilis supplementation can reduce the damage of piglets induced by ETEC K88.</p><p><strong>Methods and results: </strong>The experiment was designed with a 2 × 2 factorial arrangement, comprising the control (CON) group, Bacillus subtilis (PRO) group, Escherichia coli K88 (ETEC) group, and Bacillus subtilis + ETEC (PRO + ETEC) group. Regardless of the presence of ETEC, the addition of PRO increased the piglets' final body weight (FW), average daily gain (ADG), and daily feed intake (ADFI). Additionally, PRO primarily achieves a reduction in heat-stable enterotoxin (ST) levels, suppresses the expression of NF-κB, TLR4, and MyD88 mRNA in the jejunum and ileum, lowers pro-inflammatory factors in the blood and small intestine, enhances the expression of tight junction proteins in the small intestine, improves the composition of the colonic microbiota, increases colonic short-chain fatty acids (SCFAs) contents, thereby alleviates diarrhea and mitigates bodily damage caused by ETEC K88 infection.</p><p><strong>Conclusion: </strong>The addition of Bacillus subtilis MZ-01 alleviated ETEC K88-induced piglet diarrhoea by reducing heat-stable enterotoxin levels, decreasing pro-inflammatory factors in the blood and intestine, and enhancing the intestinal barrier and tight junction proteins.</p>","PeriodicalId":15036,"journal":{"name":"Journal of Applied Microbiology","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bacillus subtilis MZ-01 alleviates diarrhea caused by ETEC K88 by reducing inflammation and promoting intestinal health.\",\"authors\":\"Jianwei Qin, Yang Liu, Mingming Cao, Yue Zhang, Guangdong Bai, Baoming Shi\",\"doi\":\"10.1093/jambio/lxaf018\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>The purpose of this study was to investigate the effects of Bacillus subtilis supplementation on the health of weaned piglets and whether Bacillus subtilis supplementation can reduce the damage of piglets induced by ETEC K88.</p><p><strong>Methods and results: </strong>The experiment was designed with a 2 × 2 factorial arrangement, comprising the control (CON) group, Bacillus subtilis (PRO) group, Escherichia coli K88 (ETEC) group, and Bacillus subtilis + ETEC (PRO + ETEC) group. Regardless of the presence of ETEC, the addition of PRO increased the piglets' final body weight (FW), average daily gain (ADG), and daily feed intake (ADFI). Additionally, PRO primarily achieves a reduction in heat-stable enterotoxin (ST) levels, suppresses the expression of NF-κB, TLR4, and MyD88 mRNA in the jejunum and ileum, lowers pro-inflammatory factors in the blood and small intestine, enhances the expression of tight junction proteins in the small intestine, improves the composition of the colonic microbiota, increases colonic short-chain fatty acids (SCFAs) contents, thereby alleviates diarrhea and mitigates bodily damage caused by ETEC K88 infection.</p><p><strong>Conclusion: </strong>The addition of Bacillus subtilis MZ-01 alleviated ETEC K88-induced piglet diarrhoea by reducing heat-stable enterotoxin levels, decreasing pro-inflammatory factors in the blood and intestine, and enhancing the intestinal barrier and tight junction proteins.</p>\",\"PeriodicalId\":15036,\"journal\":{\"name\":\"Journal of Applied Microbiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-01-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Applied Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1093/jambio/lxaf018\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/jambio/lxaf018","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Bacillus subtilis MZ-01 alleviates diarrhea caused by ETEC K88 by reducing inflammation and promoting intestinal health.
Aims: The purpose of this study was to investigate the effects of Bacillus subtilis supplementation on the health of weaned piglets and whether Bacillus subtilis supplementation can reduce the damage of piglets induced by ETEC K88.
Methods and results: The experiment was designed with a 2 × 2 factorial arrangement, comprising the control (CON) group, Bacillus subtilis (PRO) group, Escherichia coli K88 (ETEC) group, and Bacillus subtilis + ETEC (PRO + ETEC) group. Regardless of the presence of ETEC, the addition of PRO increased the piglets' final body weight (FW), average daily gain (ADG), and daily feed intake (ADFI). Additionally, PRO primarily achieves a reduction in heat-stable enterotoxin (ST) levels, suppresses the expression of NF-κB, TLR4, and MyD88 mRNA in the jejunum and ileum, lowers pro-inflammatory factors in the blood and small intestine, enhances the expression of tight junction proteins in the small intestine, improves the composition of the colonic microbiota, increases colonic short-chain fatty acids (SCFAs) contents, thereby alleviates diarrhea and mitigates bodily damage caused by ETEC K88 infection.
Conclusion: The addition of Bacillus subtilis MZ-01 alleviated ETEC K88-induced piglet diarrhoea by reducing heat-stable enterotoxin levels, decreasing pro-inflammatory factors in the blood and intestine, and enhancing the intestinal barrier and tight junction proteins.
期刊介绍：
Journal of & Letters in Applied Microbiology are two of the flagship research journals of the Society for Applied Microbiology (SfAM). For more than 75 years they have been publishing top quality research and reviews in the broad field of applied microbiology. The journals are provided to all SfAM members as well as having a global online readership totalling more than 500,000 downloads per year in more than 200 countries. Submitting authors can expect fast decision and publication times, averaging 33 days to first decision and 34 days from acceptance to online publication. There are no page charges.
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