HSPA14在乳腺癌中的作用:肿瘤发生、免疫反应调节和个性化治疗的意义

IF 3 3区 医学 Q2 ONCOLOGY
International Journal of Hyperthermia Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI:10.1080/02656736.2025.2452922
Ruipeng Zhao, Xiaocun Zhu, Wan Wei, Linlin Zhen
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引用次数: 0

摘要

背景:热休克蛋白与癌变过程有关。HSPA14是热休克蛋白家族的一员,其在乳腺癌中的意义和病理机制尚不清楚。方法:应用TCGA数据分析HSPA14在乳腺癌组织中的表达水平及其预后意义。采用TCGA数据探讨乳腺癌患者HSPA14表达与临床病理特征的关系。GSEA分析HSPA14的生物学功能。采用Spearman相关分析检测HSPA14表达与免疫细胞浸润、免疫检查点基因的相关性。利用GSE114727的单细胞转录组学数据计算HSPA14在不同细胞亚群中的表达。HSPA14水平和药物敏感性数据提取自CellMiner数据集。通过细胞实验验证HSPA14 mRNA的表达。结果:HSPA14在乳腺癌中表达升高,与总生存率低相关。它可以作为乳腺癌患者的诊断性生物标志物。通路分析显示hspa14相关的差异基因参与细胞周期、凋亡、细胞对热应激的反应等。此外,HSPA14的表达与免疫微环境显著相关。HSPA14的表达也可能提示药物敏感性。结论:我们的研究阐明了HSPA14在肿瘤发生中的作用,特别是在调节免疫反应、塑造免疫微环境和促进耐药方面,这对乳腺癌个性化治疗的发展至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of HSPA14 in breast cancer: implications for tumorigenesis, immune response modulation, and personalized therapies.

Background: Heat shock proteins have been implicated in the process of carcinogenesis. HSPA14, a member of the heat shock protein family, remains poorly understood in terms of its significance and pathomechanisms in breast cancer.

Methods: We analyzed the expression levels of HSPA14 and its prognostic significance in breast cancer using TCGA data. TCGA data was used to investigate the association between HSPA14 expression and clinicopathological features in breast cancer patients. GSEA analysis was conducted to identify the biological function of HSPA14. Spearman's correlation analysis was performed to examine the correlation between HSPA14 expression and immune cell infiltration, as well as immune checkpoint genes. Single cell transcriptomic data from GSE114727 was utilized to calculate the expression of HSPA14 in different cell subpopulations. The data on HSPA14 levels and drug sensitivity were extracted from the CellMiner dataset. The mRNA expression of HSPA14 was validated through cell experiments.

Results: HSPA14 expression is elevated in breast cancer, which is associated with poor overall survival. It can serve as a diagnostic biomarker for breast cancer patients. Pathway analysis revealed that HSPA14-associated differential genes are involved in cell cycle, apoptosis, cellular response to heat stress, and more. Additionally, HSPA14 expression is significantly correlated with the immune microenvironment. The expression of HSPA14 may also indicate drug sensitivity.

Conclusion: Our study elucidates the involvement of HSPA14 in tumorigenesis, particularly in modulating the immune response, shaping the immune microenvironment, and contributing to drug resistance, which are pivotal for the development of personalized breast cancer therapies.

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来源期刊
CiteScore
5.90
自引率
12.90%
发文量
153
审稿时长
6-12 weeks
期刊介绍: The International Journal of Hyperthermia
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