UHPLC-MS/MS法研究mPEG5-NH2聚合物在大鼠体内的药动学行为。

IF 2.8 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography B Pub Date : 2025-02-01 DOI:10.1016/j.jchromb.2025.124460

Yingxia Guo , Meichen Liu , Jiye Tian , Chunpeng Feng , Qingbin Wang , Xuan Zhao , Lei Yin

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引用次数: 0

摘要

甲氧基聚乙二醇胺（mPEG-NH2）作为一种重要的化学试剂，在生物医学领域有着广泛的应用。阐明mPEG-NH2聚合物的药代动力学行为对于揭示mPEG-NH2相关给药系统的毒性和效率至关重要。本研究首次建立并验证了一种基于质谱（MS）的简单分析方法，用于定量生物基质中的mPEG5-NH2。分别选择m/z 252.2（前体离子）→87.7（片段离子）和m/z 371.2（前体离子）→89.2（片段离子）的多重反应监测（MRM）跃迁来测定mPEG5-NH2和OH-PEG8-OH。UHPLC-MS/MS法在0.01 ~ 10 μg/mL范围内线性良好。日内、日间准确度和精密度均在±6.44%以内。该分析方法成功地揭示了mPEG5-NH2在大鼠体内的药动学行为。

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Unraveling the in vivo pharmacokinetic behavior of mPEG5-NH2 polymer in rats by UHPLC-MS/MS assay

As an important chemical reagent, methoxy polyethylene glycol amine (mPEG-NH₂) is widely used in biomedical field. Unraveling the pharmacokinetic behavior of mPEG-NH₂ polymers is essential for revealing the toxicity and efficiency of mPEG-NH₂ related drug delivery systems. In this study, a simple analytical assay based on mass spectrometry (MS) was first established and validated for quantification of mPEG₅-NH₂ in biological matrix. The multiple reaction monitoring (MRM) transitions at m/z 252.2 (precursor ions) → 87.7 (fragment ions) and m/z 371.2 (precursor ions) → 89.2 (fragment ions) were chosen to determine mPEG₅-NH₂ and OH-PEG₈-OH, respectively. The UHPLC-MS/MS assay showed excellent linearity over the range of 0.01–10 μg/mL. Intra-day and inter-day accuracies and precisions of the assay were all within ± 6.44 %. The analytical assay was successfully applied to reveal the in vivo pharmacokinetic behavior of mPEG₅-NH₂ in rats.

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来源期刊

Journal of Chromatography B 医学-分析化学

CiteScore

5.60

自引率

3.30%

发文量

306

审稿时长

44 days

期刊介绍： The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis. Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches. Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.