鉴定底物去苏酰化酶的合成生物学方法。

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2025-01-21 DOI:10.1111/jipb.13838

Junwen Huang, Junjie Huang, Jiayuan Wu, Mi Zhou, Siyi Luo, Jieming Jiang, Tongsheng Chen, Ling Shao, Jianbin Lai, Chengwei Yang

引用次数: 0

摘要

一种在大肠杆菌中使用强大重组系统的合成生物学方法能够鉴定植物泛素样蛋白酶，该蛋白酶负责从特定蛋白质底物中去除小泛素样修饰物（SUMO）翻译后修饰。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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A synthetic biology approach for identifying de-SUMOylation enzymes of substrates.

A synthetic biology approach using a robust reconstitution system in Escherichia coli enables the identification of plant ubiquitin-like proteases responsible for removing the small ubiquitin-like modifier (SUMO) post-translational modifications from specific protein substrates.

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.