卡斯特棘阿米巴对不同来源氧化应激反应的分子机制

IF 3.8 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Proteome Research Pub Date : 2025-01-19 DOI:10.1021/acs.jproteome.4c00573

Kateřina Ženíšková, Pavel Stopka, Tania Martín-Pérez, Guillaume Chevreux, Maria Grechnikova, Eliška Drncová, Ronald Malych, Jan Mach, Julia Walochnik, Jean-Michel Camadro, Robert Sutak

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引用次数: 0

摘要

氧化应激是影响地球上所有生命的生物学原理，也是病原体-宿主关系中的一个重要因素。致病性自由生活阿米巴棘阿米巴castellanii有几种途径来应对活性氧及其造成的损害。在这项研究中，我们旨在全面分析变形虫对不同来源的氧化应激的反应。利用全细胞蛋白质组学分析，我们获得了蛋白质组变化的复杂图像，并确定了防御氧化应激的潜在关键参与者。重要的是，从差异蛋白质组学分析中，我们确定了可能参与棘阿米巴耐药的候选外排泵。

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Molecular Mechanisms of Acanthamoeba castellanii Response to Different Sources of Oxidative Stress.

Oxidative stress is a biological principle affecting all life on Earth and is also an important factor in the pathogen-host relationship. The pathogenic free-living amoeba Acanthamoeba castellanii has several pathways to cope with reactive oxygen species and the damage that they cause. In this study, we aimed to provide a comprehensive analysis of the amoeba's response to different sources of oxidative stress. Using whole-cell proteomic analysis, we obtained a complex picture of the changes in the proteome and identified potential key players in the defense against oxidative stress. Importantly, from the differential proteomics analysis, we identified a candidate efflux pump that may be involved in Acanthamoeba drug resistance.

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来源期刊

Journal of Proteome Research 生物-生化研究方法

CiteScore

9.00

自引率

4.50%

发文量

251

审稿时长

3 months

期刊介绍： Journal of Proteome Research publishes content encompassing all aspects of global protein analysis and function, including the dynamic aspects of genomics, spatio-temporal proteomics, metabonomics and metabolomics, clinical and agricultural proteomics, as well as advances in methodology including bioinformatics. The theme and emphasis is on a multidisciplinary approach to the life sciences through the synergy between the different types of "omics".