{"title":"基于皂角碳点荧光猝灭的智能手机无创唾液尿酸检测","authors":"Yujuan Xiang, Hongpeng Yu, Kegang Wu, Ziting Quan, Dong He, Cansheng Ou, Xianghua Chai, Yinglin Liang, Xuejuan Duan, Xiangyu Zhang, Qin Zha, Wei Xie","doi":"10.1007/s00604-025-06949-w","DOIUrl":null,"url":null,"abstract":"<div><p>A smartphone-based non-invasive method was developed for salivary uric acid detection using <i>Gleditsia Sinensis</i> carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Smartphone-based non-invasive detection of salivary uric acid based on the fluorescence quenching of gleditsia sinensis carbon dots\",\"authors\":\"Yujuan Xiang, Hongpeng Yu, Kegang Wu, Ziting Quan, Dong He, Cansheng Ou, Xianghua Chai, Yinglin Liang, Xuejuan Duan, Xiangyu Zhang, Qin Zha, Wei Xie\",\"doi\":\"10.1007/s00604-025-06949-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A smartphone-based non-invasive method was developed for salivary uric acid detection using <i>Gleditsia Sinensis</i> carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 2\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-01-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-06949-w\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-06949-w","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Smartphone-based non-invasive detection of salivary uric acid based on the fluorescence quenching of gleditsia sinensis carbon dots
A smartphone-based non-invasive method was developed for salivary uric acid detection using Gleditsia Sinensis carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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