无机氮和有机质共同调控外生菌根真菌介导的铁获取

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2025-01-22 DOI:10.1111/nph.20394

Haihua Wang, Kaile Zhang, Ryan Tappero, Tiffany W. Victor, Jennifer M. Bhatnagar, Rytas Vilgalys, Hui-Ling Liao

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引用次数: 0

摘要

外生菌根真菌（EMF）在促进植物从土壤中吸收养分方面起着至关重要的作用，尽管无机氮(N)可能会削弱这一作用。然而，无机氮有效性和有机质对塑造emf介导的植物铁（Fe）吸收的影响尚不清楚。为了探究这一点，我们对接种了Suillus cothurnatus的红松根系进行了微观研究，并对+/−fe包覆砂、+/−有机质和NH4NO3浓度梯度进行了处理。在有有机质而无无机氮的条件下，菌根形成最有利。对外生菌根横截面的同步x射线微荧光成像表明，无机氮对菌根铁获取的影响很大程度上取决于有机质供应。在有机质中，随着无机氮水平的增加，菌根铁浓度显著降低。相反，当有机质不存在时，观察到相反的趋势。空间分布分析表明，在所有条件下，铁、锌、钙和铜主要积聚在真菌的地幔中，这突出了地幔在养分积累和调节养分向内部隔间转移中的关键作用。我们的研究表明，土壤矿物铁的释放和emf介导的植物铁的获取受土壤无机氮和有机质的共同调节。

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Inorganic nitrogen and organic matter jointly regulate ectomycorrhizal fungi-mediated iron acquisition

Ectomycorrhizal fungi (EMF) play a crucial role in facilitating plant nutrient uptake from the soil although inorganic nitrogen (N) can potentially diminish this role. However, the effect of inorganic N availability and organic matter on shaping EMF-mediated plant iron (Fe) uptake remains unclear.
To explore this, we performed a microcosm study on Pinus taeda roots inoculated with Suillus cothurnatus treated with +/−Fe-coated sand, +/−organic matter, and a gradient of NH₄NO₃ concentrations.
Mycorrhiza formation was most favorable under conditions with organic matter, without inorganic N. Synchrotron X-ray microfluorescence imaging on ectomycorrhizal cross-sections suggested that the effect of inorganic N on mycorrhizal Fe acquisition largely depended on organic matter supply. With organic matter, mycorrhizal Fe concentration was significantly decreased as inorganic N levels increased. Conversely, an opposite trend was observed when organic matter was absent. Spatial distribution analysis showed that Fe, zinc, calcium, and copper predominantly accumulated in the fungal mantle across all conditions, highlighting the mantle's critical role in nutrient accumulation and regulation of nutrient transfer to internal compartments.
Our work illustrated that the liberation of soil mineral Fe and the EMF-mediated plant Fe acquisition are jointly regulated by inorganic N and organic matter in the soil.

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.