用小角中子散射测定α-生育酚在DPPC囊泡中的运动速率。

IF 3.2 3区生物学 Q2 BIOPHYSICS

Biophysical journal Pub Date : 2025-01-17 DOI:10.1016/j.bpj.2025.01.008

Dominik Dziura,Isabelle J Dib,Omotayo Gbadamosi,Stuart R Castillo,Maksymilian Dziura,Ryan P Murphy,Elizabeth G Kelley,Drew Marquardt

{"title":"用小角中子散射测定α-生育酚在DPPC囊泡中的运动速率。","authors":"Dominik Dziura,Isabelle J Dib,Omotayo Gbadamosi,Stuart R Castillo,Maksymilian Dziura,Ryan P Murphy,Elizabeth G Kelley,Drew Marquardt","doi":"10.1016/j.bpj.2025.01.008","DOIUrl":null,"url":null,"abstract":"α-Tocopherol (αtoc, vitamin E) is an essential nutrient sufficiently acquired through a balanced diet. This fat-soluble vitamin is most known for its antioxidative properties, however, its fundamental mechanism of action in cellular membranes remains unknown. To this end, we use time-resolved small angle neutron scattering (TR-SANS) and a contrast matching scheme to determine intervesicular exchange (kex) and intrabilayer flip-flop (kf) rates of αtoc in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles. Moreover, we investigate the role of vesicle concentration and various types of cyclodextrins in affecting these rates. For the 25 mg/mL sample concentration it was determined that kex and kf were 1.35 ± 0.03 x 10-3 min-1 and 0.54 ± 0.10 x 10-3 min-1, which represent half-lives (T1/2) of 513.4 ± 11.7 min and 1285.1 ± 242.7 min, respectively. Differential scanning calorimetry confirmed the observed timescales of αtoc movement.","PeriodicalId":8922,"journal":{"name":"Biophysical journal","volume":"37 1","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determining the Rates of α-Tocopherol Movement in DPPC Vesicles Using Small Angle Neutron Scattering.\",\"authors\":\"Dominik Dziura,Isabelle J Dib,Omotayo Gbadamosi,Stuart R Castillo,Maksymilian Dziura,Ryan P Murphy,Elizabeth G Kelley,Drew Marquardt\",\"doi\":\"10.1016/j.bpj.2025.01.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"α-Tocopherol (αtoc, vitamin E) is an essential nutrient sufficiently acquired through a balanced diet. This fat-soluble vitamin is most known for its antioxidative properties, however, its fundamental mechanism of action in cellular membranes remains unknown. To this end, we use time-resolved small angle neutron scattering (TR-SANS) and a contrast matching scheme to determine intervesicular exchange (kex) and intrabilayer flip-flop (kf) rates of αtoc in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles. Moreover, we investigate the role of vesicle concentration and various types of cyclodextrins in affecting these rates. For the 25 mg/mL sample concentration it was determined that kex and kf were 1.35 ± 0.03 x 10-3 min-1 and 0.54 ± 0.10 x 10-3 min-1, which represent half-lives (T1/2) of 513.4 ± 11.7 min and 1285.1 ± 242.7 min, respectively. Differential scanning calorimetry confirmed the observed timescales of αtoc movement.\",\"PeriodicalId\":8922,\"journal\":{\"name\":\"Biophysical journal\",\"volume\":\"37 1\",\"pages\":\"\"},\"PeriodicalIF\":3.2000,\"publicationDate\":\"2025-01-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biophysical journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.bpj.2025.01.008\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biophysical journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.bpj.2025.01.008","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

摘要

α-生育酚（α - toc，维生素E）是通过均衡饮食获得的必需营养素。这种脂溶性维生素以其抗氧化特性而闻名，然而，其在细胞膜中的基本作用机制尚不清楚。为此，我们使用时间分辨小角中子散射（r - sans）和对比匹配方案来测定1,2-二棕榈酰-sn-甘油-3-磷脂胆碱（DPPC）囊泡中α - toc的囊间交换（kex）和囊内触发器（kf）速率。此外，我们还研究了囊泡浓度和不同类型的环糊精对这些速率的影响。在25 mg/mL样品浓度下，kex和kf分别为1.35±0.03 × 10-3 min-1和0.54±0.10 × 10-3 min-1，半衰期（T1/2）分别为513.4±11.7 min和1285.1±242.7 min。差示扫描量热法证实了α - toc运动的时间尺度。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Determining the Rates of α-Tocopherol Movement in DPPC Vesicles Using Small Angle Neutron Scattering.

α-Tocopherol (αtoc, vitamin E) is an essential nutrient sufficiently acquired through a balanced diet. This fat-soluble vitamin is most known for its antioxidative properties, however, its fundamental mechanism of action in cellular membranes remains unknown. To this end, we use time-resolved small angle neutron scattering (TR-SANS) and a contrast matching scheme to determine intervesicular exchange (kex) and intrabilayer flip-flop (kf) rates of αtoc in 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) vesicles. Moreover, we investigate the role of vesicle concentration and various types of cyclodextrins in affecting these rates. For the 25 mg/mL sample concentration it was determined that kex and kf were 1.35 ± 0.03 x 10-3 min-1 and 0.54 ± 0.10 x 10-3 min-1, which represent half-lives (T1/2) of 513.4 ± 11.7 min and 1285.1 ± 242.7 min, respectively. Differential scanning calorimetry confirmed the observed timescales of αtoc movement.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biophysical journal 生物-生物物理

CiteScore

6.10

自引率

5.90%

发文量

3090

审稿时长

2 months

期刊介绍： BJ publishes original articles, letters, and perspectives on important problems in modern biophysics. The papers should be written so as to be of interest to a broad community of biophysicists. BJ welcomes experimental studies that employ quantitative physical approaches for the study of biological systems, including or spanning scales from molecule to whole organism. Experimental studies of a purely descriptive or phenomenological nature, with no theoretical or mechanistic underpinning, are not appropriate for publication in BJ. Theoretical studies should offer new insights into the understanding ofexperimental results or suggest new experimentally testable hypotheses. Articles reporting significant methodological or technological advances, which have potential to open new areas of biophysical investigation, are also suitable for publication in BJ. Papers describing improvements in accuracy or speed of existing methods or extra detail within methods described previously are not suitable for BJ.