基于铱基自增强纳米发射器的多信号放大策略制备用于检测阿尔茨海默病生物标志物的超灵敏电化学发光磁免疫传感器

IF 9.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2025-01-21 DOI:10.1021/acssensors.4c02916

Chenji Dai, Yaoyao Xu, Libing Ke, Mengjiao Zhu, Rongxiu Deng, Xuedong Wang, Yuyang Zhou

{"title":"基于铱基自增强纳米发射器的多信号放大策略制备用于检测阿尔茨海默病生物标志物的超灵敏电化学发光磁免疫传感器","authors":"Chenji Dai, Yaoyao Xu, Libing Ke, Mengjiao Zhu, Rongxiu Deng, Xuedong Wang, Yuyang Zhou","doi":"10.1021/acssensors.4c02916","DOIUrl":null,"url":null,"abstract":"Alzheimer’s disease (AD) is characterized by progressive memory loss and cognitive decline, significantly impairing the daily life of elderly individuals. The low abundance of blood-based biomarkers in AD necessitates higher analytical technique requirements. Herein, one novel iridium-based ECL self-enhanced nanoemitter (TPrA@Ir-SiO2) was unprecedentedly reported, and it was further used to construct an ultrasensitive ECL magnetic immunosensor by a multiple-signal amplification strategy to unequally sensitively and accurately detect the AD blood-based biomarker (P-tau181) in this work. The initial signal amplification was accomplished via incorporating a new efficient iridium-based luminophore named Ir(mdq)2(acac) and a corresponding coreactant into silica nanoparticles to successfully obtain TPrA@Ir-SiO2. In addition, the specific and high-affinity interactions between streptavidin and biotin were subsequently employed to further facilitate signal amplification. Based on the advantages of the luminophore itself and the high-affinity interactions between biotin and streptavidin, the corresponding ECL immunosensor proposed in this work exhibited remarkable sensitivity, covering a wide linear range from 0.1 pg/mL to 0.1 μg/mL, and achieved an ultralow limit of detection of 68.58 fg/mL (S/N = 3), and it also exhibited outstanding recovery (98–104%) and RSD (1.92–4.86%) in the detection of serum samples by the spiking method. These remarkable results undoubtedly demonstrate the potential of self-enhanced ECL nanoemitters combined with a synergistic signal amplification strategy bearing streptavidin–biotin in detecting AD blood-based biomarkers, providing accurate and reliable solutions for early diagnosis and monitoring of AD, which would open a new avenue to effectively reduce the burden on AD patients’ families and society in the future.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"37 1","pages":""},"PeriodicalIF":9.1000,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Multiple-Signal Amplification Strategy to Fabricate an Ultrasensitive Electrochemiluminescence Magnetic Immunosensor for Detecting Biomarkers of Alzheimer’s Disease via Iridium-Based Self-Enhancing Nanoemitters\",\"authors\":\"Chenji Dai, Yaoyao Xu, Libing Ke, Mengjiao Zhu, Rongxiu Deng, Xuedong Wang, Yuyang Zhou\",\"doi\":\"10.1021/acssensors.4c02916\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Alzheimer’s disease (AD) is characterized by progressive memory loss and cognitive decline, significantly impairing the daily life of elderly individuals. The low abundance of blood-based biomarkers in AD necessitates higher analytical technique requirements. Herein, one novel iridium-based ECL self-enhanced nanoemitter (TPrA@Ir-SiO2) was unprecedentedly reported, and it was further used to construct an ultrasensitive ECL magnetic immunosensor by a multiple-signal amplification strategy to unequally sensitively and accurately detect the AD blood-based biomarker (P-tau181) in this work. The initial signal amplification was accomplished via incorporating a new efficient iridium-based luminophore named Ir(mdq)2(acac) and a corresponding coreactant into silica nanoparticles to successfully obtain TPrA@Ir-SiO2. In addition, the specific and high-affinity interactions between streptavidin and biotin were subsequently employed to further facilitate signal amplification. Based on the advantages of the luminophore itself and the high-affinity interactions between biotin and streptavidin, the corresponding ECL immunosensor proposed in this work exhibited remarkable sensitivity, covering a wide linear range from 0.1 pg/mL to 0.1 μg/mL, and achieved an ultralow limit of detection of 68.58 fg/mL (S/N = 3), and it also exhibited outstanding recovery (98–104%) and RSD (1.92–4.86%) in the detection of serum samples by the spiking method. These remarkable results undoubtedly demonstrate the potential of self-enhanced ECL nanoemitters combined with a synergistic signal amplification strategy bearing streptavidin–biotin in detecting AD blood-based biomarkers, providing accurate and reliable solutions for early diagnosis and monitoring of AD, which would open a new avenue to effectively reduce the burden on AD patients’ families and society in the future.\",\"PeriodicalId\":24,\"journal\":{\"name\":\"ACS Sensors\",\"volume\":\"37 1\",\"pages\":\"\"},\"PeriodicalIF\":9.1000,\"publicationDate\":\"2025-01-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Sensors\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.1021/acssensors.4c02916\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Sensors","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acssensors.4c02916","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

摘要

阿尔茨海默病（AD）的特点是进行性记忆丧失和认知能力下降，严重损害老年人的日常生活。阿尔茨海默病血液生物标志物的低丰度要求更高的分析技术。本文首次报道了一种新的基于铱的ECL自增强纳米发射器（TPrA@Ir-SiO2），并利用该纳米发射器构建了一种超灵敏的ECL磁免疫传感器，通过多信号放大策略对AD血液生物标志物（P-tau181）进行非等灵敏度和准确性检测。最初的信号放大是通过将一种名为Ir(mdq)2（acac）的新型高效铱基发光团和相应的助反应物加入二氧化硅纳米颗粒中来完成的，从而成功地获得TPrA@Ir-SiO2。此外，随后利用链霉亲和素与生物素之间的特异性和高亲和力相互作用进一步促进信号放大。基于发光团本身的优势以及生物素与链霉亲和素之间的高亲和相互作用，本文所构建的ECL免疫传感器具有显著的灵敏度，在0.1 pg/mL ~ 0.1 μg/mL的宽线性范围内，达到了68.58 fg/mL的超低检出限（S/N = 3），并且在峰化法检测血清样品中具有出色的回收率（98 ~ 104%）和RSD（1.92 ~ 4.86%）。这些显著的结果无疑证明了自增强ECL纳米发射体结合链亲和素-生物素协同信号放大策略在检测AD血液生物标志物方面的潜力，为AD的早期诊断和监测提供了准确可靠的解决方案，这将为未来有效减轻AD患者家庭和社会的负担开辟新的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Multiple-Signal Amplification Strategy to Fabricate an Ultrasensitive Electrochemiluminescence Magnetic Immunosensor for Detecting Biomarkers of Alzheimer’s Disease via Iridium-Based Self-Enhancing Nanoemitters

查看原文本刊更多论文

Alzheimer’s disease (AD) is characterized by progressive memory loss and cognitive decline, significantly impairing the daily life of elderly individuals. The low abundance of blood-based biomarkers in AD necessitates higher analytical technique requirements. Herein, one novel iridium-based ECL self-enhanced nanoemitter (TPrA@Ir-SiO₂) was unprecedentedly reported, and it was further used to construct an ultrasensitive ECL magnetic immunosensor by a multiple-signal amplification strategy to unequally sensitively and accurately detect the AD blood-based biomarker (P-tau181) in this work. The initial signal amplification was accomplished via incorporating a new efficient iridium-based luminophore named Ir(mdq)₂(acac) and a corresponding coreactant into silica nanoparticles to successfully obtain TPrA@Ir-SiO₂. In addition, the specific and high-affinity interactions between streptavidin and biotin were subsequently employed to further facilitate signal amplification. Based on the advantages of the luminophore itself and the high-affinity interactions between biotin and streptavidin, the corresponding ECL immunosensor proposed in this work exhibited remarkable sensitivity, covering a wide linear range from 0.1 pg/mL to 0.1 μg/mL, and achieved an ultralow limit of detection of 68.58 fg/mL (S/N = 3), and it also exhibited outstanding recovery (98–104%) and RSD (1.92–4.86%) in the detection of serum samples by the spiking method. These remarkable results undoubtedly demonstrate the potential of self-enhanced ECL nanoemitters combined with a synergistic signal amplification strategy bearing streptavidin–biotin in detecting AD blood-based biomarkers, providing accurate and reliable solutions for early diagnosis and monitoring of AD, which would open a new avenue to effectively reduce the burden on AD patients’ families and society in the future.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.