开发空间生物制造的替代介质

IF 15.7 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Hakyung Lee, Jinjin Diao, Yuxin Tian, Richa Guleria, Eunseo Lee, Alexandra Smith, Millie Savage, Daniel Yeh, Luke Roberson, Mark Blenner, Yinjie J. Tang, Tae Seok Moon
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引用次数: 0

摘要

太空生物制造提供了一种可持续的解决方案,以促进人类在地外环境中长期、自给自足的居住。然而,它对地球供应的原料的依赖使得太空生物制造在经济上不可行。在这里,我们开发了一种称为替代原料驱动的原位生物制造(AF-ISM)的过程,以减轻对地球原料再供应的依赖。具体来说,我们研究了三种替代原料(AF)——火星和月球风化层、消费后聚对苯二甲酸乙二醇酯和粪便废物,以开发一种利用约氏红球菌PET菌株S6 (RPET S6)生产番茄红素的替代培养基。结果表明,RPET S6可以直接利用模拟风化层颗粒作为矿物替代品,而添加厌氧预处理的粪便可协同支持其细胞生长。此外,在微重力条件下使用AF生产番茄红素达到了与地球相当的水平。此外,经济分析表明,与传统方法相比,使用AF-ISM可以显著降低番茄红素的生产成本。总的来说,这项工作突出了AF-ISM在空间生物制造中的可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Developing an alternative medium for in-space biomanufacturing

Developing an alternative medium for in-space biomanufacturing

In-space biomanufacturing provides a sustainable solution to facilitate long-term, self-sufficient human habitation in extraterrestrial environments. However, its dependence on Earth-supplied feedstocks renders in-space biomanufacturing economically nonviable. Here, we develop a process termed alternative feedstock-driven in-situ biomanufacturing (AF-ISM) to alleviate dependence on Earth-based resupply of feedstocks. Specifically, we investigate three alternative feedstocks (AF)—Martian and Lunar regolith, post-consumer polyethylene terephthalate, and fecal waste—to develop an alternative medium for lycopene production using Rhodococcus jostii PET strain S6 (RPET S6). Our results show that RPET S6 could directly utilize regolith simulant particles as mineral replacements, while the addition of anaerobically pretreated fecal waste synergistically supported its cell growth. Additionally, lycopene production using AF under microgravity conditions achieved levels comparable to those on Earth. Furthermore, an economic analysis shows significant lycopene production cost reductions using AF-ISM versus conventional methods. Overall, this work highlights the viability of AF-ISM for in-space biomanufacturing.

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来源期刊
Nature Communications
Nature Communications Biological Science Disciplines-
CiteScore
24.90
自引率
2.40%
发文量
6928
审稿时长
3.7 months
期刊介绍: Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.
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