RNA剪接：一个分裂共识揭示了两个主要的5'剪接位点类别。

IF 4.5 3区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Open Biology Pub Date : 2025-01-01 Epub Date: 2025-01-15 DOI:10.1098/rsob.240293

Matthew T Parker, Sebastian M Fica, Gordon G Simpson

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引用次数: 0

摘要

已建立的人类5'剪接位点的共识序列掩盖了由U5 snRNA环1或U6 snRNA ACAGA盒的优先碱基配对潜力定义的两种主要剪接位点类别的存在。这两种5'剪接位点在基因组序列中是可分离的，受特定基因型的影响，并与剪接复杂性有关。这两个类别反映了5‘剪接位点的使用主要发生在5’剪接位点转移到U6 snRNA的过程中。将人类5'剪接位点共识分解为两个主要成分，可以帮助我们了解真核生物基因组结构和剪接机制的基本特征，并为影响剪接的遗传变异引起的疾病的治疗设计提供信息。

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RNA splicing: a split consensus reveals two major 5' splice site classes.

The established consensus sequence for human 5' splice sites masks the presence of two major splice site classes defined by preferential base-pairing potentials with either U5 snRNA loop 1 or the U6 snRNA ACAGA box. The two 5' splice site classes are separable in genome sequences, sensitized by specific genotypes and associated with splicing complexity. The two classes reflect the commitment to 5' splice site usage occurring primarily during 5' splice site transfer to U6 snRNA. Separating the human 5' splice site consensus into its two major constituents can help us understand fundamental features of eukaryote genome architecture and splicing mechanisms and inform treatment design for diseases caused by genetic variation affecting splicing.

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来源期刊

Open Biology BIOCHEMISTRY & MOLECULAR BIOLOGY-

CiteScore

10.00

自引率

1.70%

发文量

136

审稿时长

6-12 weeks

期刊介绍： Open Biology is an online journal that welcomes original, high impact research in cell and developmental biology, molecular and structural biology, biochemistry, neuroscience, immunology, microbiology and genetics.