基于微磨液滴微流体的元件库创建和像素阵列生成。

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
David McIntyre, Diana Arguijo, Kaede Kawata, Douglas Densmore
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引用次数: 0

摘要

微滴微流体使高通量筛选,测序和制剂的生物和化学系统在微尺度。这种装置通常在软聚合物如聚二甲基硅氧烷(PDMS)中制造。然而,为PDMS设备开发设计掩模可能是一个缓慢而昂贵的过程,需要内部洁净室设施或使用外部供应商。在这里,我们提出了第一个完整的基于液滴的组件库,使用低成本的快速原型和电极集成。这种液滴微流体装置的制造方法每个装置的成本不到12美元,并且可以在一天内完成完整的设计-制造-测试周期。构建并表征了用于液滴生成、回注、皮注射、锚定、荧光传感和分选的离散微流体组件。这些装置具有生物相容性、低成本和高通量。为了展示其执行多步骤工作流程的能力,这些组件用于组装液滴“像素”阵列,其中液滴被生成、感测、排序并固定在网格上以生成图像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Component library creation and pixel array generation with micromilled droplet microfluidics.

Droplet microfluidics enable high-throughput screening, sequencing, and formulation of biological and chemical systems at the microscale. Such devices are generally fabricated in a soft polymer such as polydimethylsiloxane (PDMS). However, developing design masks for PDMS devices can be a slow and expensive process, requiring an internal cleanroom facility or using an external vendor. Here, we present the first complete droplet-based component library using low-cost rapid prototyping and electrode integration. This fabrication method for droplet microfluidic devices costs less than $12 per device and a full design-build-test cycle can be completed within a day. Discrete microfluidic components for droplet generation, re-injection, picoinjection, anchoring, fluorescence sensing, and sorting were built and characterized. These devices are biocompatible, low-cost, and high-throughput. To show its ability to perform multistep workflows, these components were used to assemble droplet "pixel" arrays, where droplets were generated, sensed, sorted, and anchored onto a grid to produce images.

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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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