高表达的CYP6CY1基因参与了稻蛾对吡虫啉的抗性。

IF 1.4 3区农林科学 Q2 ENTOMOLOGY

Neotropical Entomology Pub Date : 2025-01-14 DOI:10.1007/s13744-024-01239-1

Yun-Shan Xiong, Ling-Ling Cui, Gui-Lei Hu, Yu-Tai Jiang, Yong-Po Lv, Pei Zhang, Jin-Song Zheng, Bai-Zhong Zhang, Run-Qiang Liu

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引用次数: 0

摘要

小麦蚜虫是一种严重危害农业生产的小麦蚜虫。小麦蚜虫的有效治理一直依赖于化学杀虫剂。然而，它们的广泛应用导致小麦蚜虫对某些杀虫剂产生严重抗性，而细胞色素P450作为一种解毒酶在小麦蚜虫对杀虫剂产生抗性的过程中起着至关重要的作用。本研究分离到了一个新的P450基因CYP6CY1，并在吡虫啉耐药菌株中过表达。CYP6CY1基因沉默后对吡虫啉敏感性增加，提示其参与吡虫啉耐药。随后，在转录后调控水平上证实了CYP6CY1的3' UTR内miR-3047-3p的作用，并被证明参与了吡虫啉的耐药。这些结果为了解P450在害虫杀虫剂抗性中的作用提供了机会。

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Overexpression of CYP6CY1 is Involved in Imidacloprid Resistance in Sitobion miscanthi (Takahashi) (Homoptera: Aphidae).

Sitobion miscanthi is a wheat aphid species that can damage seriously agricultural production. The effective management of wheat aphids has depended on chemical insecticides. However, their wide application led to severe resistance of wheat aphids to some insecticides, and cytochrome P450 as a detoxifying enzyme plays a crucial role in the insecticide resistance. In this study, CYP6CY1, a new P450 gene, was isolated and overexpressed in a S.miscanthi resistant strain to imidacloprid. The increased sensitivity to imidacloprid after silencing of CYP6CY1 indicated that its involvement in imidacloprid resistance. Subsequently, action of miR-3047-3p within the 3' UTR of the CYP6CY1 was confirmed at the posttranscriptional regulatory level, and was shown to be involved in the resistance to imidacloprid. These results provide opportunities for understanding the roles of P450 in insecticide resistance of pests.

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来源期刊

Neotropical Entomology 生物-昆虫学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： Neotropical Entomology is a bimonthly journal, edited by the Sociedade Entomológica do Brasil (Entomological Society of Brazil) that publishes original articles produced by Brazilian and international experts in several subspecialties of entomology. These include bionomics, systematics, morphology, physiology, behavior, ecology, biological control, crop protection and acarology.