富血小板血浆对Erastin诱导的髓核细胞凋亡的影响。

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-12-24 DOI:10.1016/j.bbrep.2024.101900

Shi-lin Lian , Jie Huang , Yan Zhang , Yu Ding

{"title":"富血小板血浆对Erastin诱导的髓核细胞凋亡的影响。","authors":"Shi-lin Lian , Jie Huang , Yan Zhang , Yu Ding","doi":"10.1016/j.bbrep.2024.101900","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear.</div></div><div><h3>Purpose</h3><div>This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin.</div></div><div><h3>Methods</h3><div>Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM).</div></div><div><h3>Results</h3><div>PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P < 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P < 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P < 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P < 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P < 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery.</div></div><div><h3>Conclusions</h3><div>The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"41 ","pages":"Article 101900"},"PeriodicalIF":2.3000,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732229/pdf/","citationCount":"0","resultStr":"{\"title\":\"The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin\",\"authors\":\"Shi-lin Lian , Jie Huang , Yan Zhang , Yu Ding\",\"doi\":\"10.1016/j.bbrep.2024.101900\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear.</div></div><div><h3>Purpose</h3><div>This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin.</div></div><div><h3>Methods</h3><div>Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM).</div></div><div><h3>Results</h3><div>PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P < 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P < 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P < 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P < 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P < 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery.</div></div><div><h3>Conclusions</h3><div>The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases.</div></div>\",\"PeriodicalId\":8771,\"journal\":{\"name\":\"Biochemistry and Biophysics Reports\",\"volume\":\"41 \",\"pages\":\"Article 101900\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-12-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732229/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochemistry and Biophysics Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405580824002644\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580824002644","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

背景：椎间盘退变（IVDD）与铁下垂（一种程序性细胞死亡）有关。富血小板血浆（PRP）在IVDD中减轻髓核（NP）细胞铁下垂中的作用尚不清楚。目的：验证PRP对Erastin诱导的NP细胞铁凋亡的影响。方法：分离SD大鼠原代NP细胞，用Erastin建立铁凋亡模型。制备PRP并应用于评估其对铁中毒相关标志物的影响，包括活性氧（ROS）、铁含量和谷胱甘肽过氧化物酶4 （GPX4）。透射电镜观察了PRP对NP细胞超微结构的影响。结果：PRP治疗可显著恢复GPX4水平（431.47±4.70 ng/L vs 69.70±4.06 ng/L， P）。结论：本研究通过减轻NP细胞铁下垂，证明了PRP治疗IVDD的潜力，为PRP治疗退变性椎间盘疾病提供了新的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The effect of platelet-rich plasma on ferroptosis of nucleus pulposus cells induced by Erastin

Background

Intervertebral disc degeneration (IVDD) has been linked to ferroptosis, a type of programmed cell death. The role of platelet-rich plasma (PRP) in mitigating ferroptosis in nucleus pulposus (NP) cells within IVDD remains unclear.

Purpose

This study aims to verify the effectiveness of PRP in reducing ferroptosis in NP cells induced by Erastin.

Methods

Primary NP cells were isolated from SD rats, and a ferroptosis model was established using Erastin. PRP was prepared and applied to assess its impact on ferroptosis-related markers, including reactive oxygen species (ROS), iron content, and glutathione peroxidase 4 (GPX4). The effects of PRP on the ultrastructure of NP cells were also observed using transmission electron microscopy (TEM).

Results

PRP treatment significantly restored GPX4 levels (431.47 ± 4.70 ng/L vs. 69.70 ± 4.06 ng/L, P < 0.05), reduced ROS levels (45.06 ± 3.78 vs. 155.36 ± 3.56, P < 0.05), and decreased iron content (32.24 ± 096 μg/L vs. 59.25 ± 3.72 μg/L, P < 0.05) in ferroptotic NP cells compared to the sham group. Additionally, PRP significantly increased the expression levels of collagen Ⅱ (0.72 ± 0.02 vs. 0.33 ± 0.02, P < 0.05) and aggrecan (0.81 ± 0.01 vs. 0.31 ± 0.02, P < 0.05) compared to the sham group. TEM analysis also showed improvements in mitochondrial ultrastructure. These findings suggest that PRP can alleviate ferroptosis and promote cellular recovery.

Conclusions

The study demonstrates the potential of PRP as a therapeutic intervention in IVDD by mitigating ferroptosis in NP cells, offering a new theoretical basis for PRP treatment in degenerative disc diseases.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.