Nurimangul Muntiza, Wenbin Zhang, Yuzeng Li, Wenquan Ji, Jin Zhao, Hongfeng Zhang, Qinran Li, Qiliang Deng, Donglan Sun, Tianjun Liu
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After cucurbit[6]uril modification and epitope immobilization, the imprinted polymer was synthesized by RAFT polymerization with 2-dodecylsulfanylcarbothioylsulfanyl-2-methylpropanoic acid as chain transfer agent. After template removal, the obtained imprinted particles showed good binding ability to GI-9 (20.28 mg g<sup>-1</sup>, IF = 4.11) and cytochrome c (36.12 mg g<sup>-1</sup>, IF = 3.91). With the successive addition of cucurbit[6]uril and RAFT agent, the step-by-step improvement of the IF for cytochrome c recognition further illustrated the effects of supramolecular host-guest interaction and regulation of imprinted polymer chain. The imprinted polymers showed obvious advantages for cytochrome c recognition compared to competitive proteins and had good reusability with the repeated reproduction rate 80.8 % after five cycles of adsorption and desorption. Furthermore, the selective recognition for cytochrome c in adult bovine serum proved its potentiality to be applied in practical samples. All these results demonstrated that the combination of epitope imprinting, cucurbit[6]uril host-guest interaction and RAFT strategy presented an efficient new feasible control method for protein recognition with good selectivity, stability and reusability.</p>","PeriodicalId":435,"journal":{"name":"Talanta","volume":"286 ","pages":"127567"},"PeriodicalIF":5.6000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cucurbit[6]uril host-guest interaction assisted N-terminal epitope imprinted particles for cytochrome c recognition prepared by reversible addition-fragmentation chain transfer strategy.\",\"authors\":\"Nurimangul Muntiza, Wenbin Zhang, Yuzeng Li, Wenquan Ji, Jin Zhao, Hongfeng Zhang, Qinran Li, Qiliang Deng, Donglan Sun, Tianjun Liu\",\"doi\":\"10.1016/j.talanta.2025.127567\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A novel strategy for cytochrome c selective recognition assisted with cucurbit[6]uril by host-guest interaction via N-terminal epitope imprinting and reversible addition-fragmentation chain transfer (RAFT) polymerization was developed. 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引用次数: 0
摘要
提出了一种通过n端表位印迹和可逆加成-片段链转移（RAFT）聚合的宿主-客体相互作用辅助细胞色素c选择性识别的新策略。以细胞色素c的n端非肽（GI-9）作为表位模板，实现对细胞色素c的高选择性识别。作为近年来常见的超分子，葫芦[6]uril可以包封赖氨酸残基的丁铵基来捕获肽，并通过主客体相互作用提高相应的空间取向，从而实现对GI-9或细胞色素c的识别。以2-十二烷基磺酰碳硫基磺酰-2-甲基丙烷酸为链转移剂，通过RAFT聚合法制备印迹聚合物。模板去除后，印迹颗粒对GI-9 （20.28 mg g-1, IF = 4.11）和细胞色素c （36.12 mg g-1, IF = 3.91）具有良好的结合能力。随着瓜b[6]uril和RAFT试剂的相继加入，IF对细胞色素c识别的逐步提高进一步说明了超分子主客体相互作用和印迹聚合物链调控的作用。印迹聚合物对细胞色素c的识别能力明显优于竞争蛋白，并且具有良好的重复利用性，经过5次吸附和解吸后，重复繁殖率达到80.8%。此外，对成年牛血清中细胞色素c的选择性识别也证明了该方法在实际样品中的应用潜力。这些结果表明，表位印迹、瓜bbbbil宿主-客体相互作用和RAFT策略相结合是一种高效可行的蛋白质识别控制新方法，具有良好的选择性、稳定性和可重用性。
Cucurbit[6]uril host-guest interaction assisted N-terminal epitope imprinted particles for cytochrome c recognition prepared by reversible addition-fragmentation chain transfer strategy.
A novel strategy for cytochrome c selective recognition assisted with cucurbit[6]uril by host-guest interaction via N-terminal epitope imprinting and reversible addition-fragmentation chain transfer (RAFT) polymerization was developed. N-terminal nonapeptide of cytochrome c (GI-9) was used as the epitope template to achieve highly selective recognition of cytochrome c. As a common supramolecule in recent years, cucurbit[6]uril can encapsulate the butyrammonium group of lysine residue to capture the peptide and improve the corresponding spatial orientation by the host-guest interaction for GI-9 or cytochrome c recognition. After cucurbit[6]uril modification and epitope immobilization, the imprinted polymer was synthesized by RAFT polymerization with 2-dodecylsulfanylcarbothioylsulfanyl-2-methylpropanoic acid as chain transfer agent. After template removal, the obtained imprinted particles showed good binding ability to GI-9 (20.28 mg g-1, IF = 4.11) and cytochrome c (36.12 mg g-1, IF = 3.91). With the successive addition of cucurbit[6]uril and RAFT agent, the step-by-step improvement of the IF for cytochrome c recognition further illustrated the effects of supramolecular host-guest interaction and regulation of imprinted polymer chain. The imprinted polymers showed obvious advantages for cytochrome c recognition compared to competitive proteins and had good reusability with the repeated reproduction rate 80.8 % after five cycles of adsorption and desorption. Furthermore, the selective recognition for cytochrome c in adult bovine serum proved its potentiality to be applied in practical samples. All these results demonstrated that the combination of epitope imprinting, cucurbit[6]uril host-guest interaction and RAFT strategy presented an efficient new feasible control method for protein recognition with good selectivity, stability and reusability.
期刊介绍：
Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome.
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