Electrospun Chitosan/Polylactic Acid Nanofibers with Silver Nanoparticles: Structure, Antibacterial, and Cytotoxic Properties.

Electrospinning, a technique for creating fabric materials from polymer solutions, is widely used in various fields, including biomedicine. The unique properties of electrospun fibrous membranes, such as large surface area, compositional versatility, and customizable porous structure, make them ideal for advanced biomedical applications like tissue engineering and wound healing. By considering the high biocompatibility and well-known regenerative potential of polylactic acid (PLA) and chitosan (CH), as well as the versatile antibacterial effect of silver nanoparticles (AgNPs), this study explores the antibacterial efficacy, adhesive properties, and cytotoxicity of electrospun chitosan membranes with a unique nanofibrous structure and varying concentrations of AgNPs. Silver nanoparticles incorporated at concentrations of 25-50 μg/mL or above significantly enhanced the antibacterial effectiveness, especially against Staphylococcus aureus and Escherichia coli. Biocompatibility assessments using umbilical cord mesenchymal stem cells demonstrated the nontoxic nature of the membranes with an AgNP concentration of 12.5 μg/mL, underscoring their potential for biomedical applications. This study provides valuable insights into developing electrospun chitosan membranes as effective antimicrobial coatings for various biomedical uses, including wound healing patches and tissue engineering constructs for soft tissue replacement.