[基于外观性状和内在质量指标的板蓝根饮片质量分级标准的建立]。

Q3 Pharmacology, Toxicology and Pharmaceutics
Yan Li, Kang-Ning Fu, Shuai Wang, Li Yang, Zheng-Tao Wang, Rui Wang, Yan-Hong Shi
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引用次数: 0

摘要

本研究旨在建立一种将传统的基于形态特征的中药质量评价与现代质量评价相结合的质量分级标准。根据板蓝根现有标准和市场流通情况,选取板蓝根饮片的直径和颜色作为外观性状进行初步分级。综合比较水分、总灰分、酸不溶灰分、醇溶浸出物、9种水溶性组分等内部品质指标对不同等级饮片的影响,并通过t检验确定关键分级指标。采用相关分析、主成分分析(PCA)、正交偏最小二乘判别分析(OPLS-DA)和层次聚类分析(HCA)等多维统计分析方法,研究了外观性状与内在品质指标的相关性。采用荧光底物研究不同等级板蓝根饮片对流感病毒神经氨酸酶的抑制作用。通过网络药理学验证所选分级指标成分的合理性。结果表明:饮片直径与除水分外的其他指标组分呈显著正相关。结合板蓝根现有的商品规格、分级标准和市场流通实际,以直径为外观特征,(R,S)-甲状腺素为合格的限定指标成分,制定了板蓝根饮片的质量分级标准。在此基础上,选取醇溶性提取物、总紫荆素和总核苷的含量进行质量分级。体外酶活性评价表明,一级板蓝根饮片对流感病毒的神经氨酸酶有较强的抑制作用,网络药理学结果表明,质量分级所选择的指标成分与流感有显著相关性。本研究建立了结合外观性状和内在质量指标的板蓝根饮片质量分级标准,为完善现有分级标准及板蓝根饮片的价格和质量提供了科学依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Establishment of a quality grading standard of Isatidis Radix decoction pieces based on appearance traits and internal quality indexes].

This study aims to establish a quality grading standard that combines the conventional quality evaluation based on morphological characteristics of traditional Chinese medicine with the modern quality evaluation. Based on the existing standards and market circulation of Isatidis Radix, the diameter and color of Isatidis Radix decoction pieces were selected as the appearance traits for preliminary grading. The effects of internal quality indexes such as moisture, total ash, acid-insoluble ash, ethanol-soluble extractives, and 9 water-soluble components on different grades of decoction pieces were comprehensively compared, and the key grading indexes were determined by t-test. The relevance between appearance traits and the internal quality indexes was investigated by multi-dimensional statistical analyses such as correlation analysis, principal component analysis(PCA), orthogonal partial least squares-discriminant analysis(OPLS-DA), and hierarchical cluster analysis(HCA). Furthermore, the fluorescent substrate was employed to investigate the inhibitory effects of different grades of Isatidis Radix decoction pieces on the neuraminidase of influenza virus. The rationality of the selected grading index components was verified by network pharmacology. The results showed that the diameter of decoction pieces had significantly positive correlations with other index components except moisture. Considering the existing commodity specifications, grading standards, and actual market circulation of Isatidis Radix, the quality grading standard of Isatidis Radix decoction pieces was developed with diameter as the appearance trait and(R,S)-goitrin as the limit index component of being qualified. On this basis, the content of ethanol-soluble extractives, total sinigrin, and total nucleoside were selected for quality grading. The in vitro enzyme activity evaluation showed that the first grade of Isatidis Radix decoction pieces had stronger inhibitory effect on the neuraminidase of influenza virus, and the network pharmacology results showed that the selected index components for quality grading had significant correlations with influenza. This study established a quality grading standard combining appearance traits and internal quality indexes, which provides a scientific basis for improving the existing grading standards and the price and quality of Isatidis Radix decoction pieces.

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来源期刊
Zhongguo Zhongyao Zazhi
Zhongguo Zhongyao Zazhi Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.50
自引率
0.00%
发文量
581
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