拟南芥本地自然种群花粉杀手新基因的鉴定。

IF 4 2区 生物学 Q1 GENETICS & HEREDITY
PLoS Genetics Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI:10.1371/journal.pgen.1011451
Anthony Ricou, Matthieu Simon, Rémi Duflos, Marianne Azzopardi, Fabrice Roux, Françoise Budar, Christine Camilleri
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引用次数: 0

摘要

配子杀手是扭曲杂交后代分离的基因位点,因为杀手等位基因促进了携带敏感等位基因的配子的消除。它们广泛分布于真核生物中,对了解基因组进化和物种形成具有重要意义。我们以前已经在两个遥远的自然加入的拟南芥杂种中发现了花粉杀手。这种花粉杀手涉及三个遗传上相关的基因,我们之前报道了编码保护花粉粒免受杀手活动的解毒剂的基因的鉴定。在本研究中,我们利用CRISPR-Cas9诱导突变体鉴定了花粉杀手的另外两个基因。这两个基因对于我们所证明的针对花粉的杀伤活性是必要的。花粉杀手编码蛋白的细胞定位表明,花粉杀手活性涉及线粒体。序列分析揭示了杀手蛋白中来自相同家族的预测结构域。此外,其中一种杀伤蛋白的c端一半与解毒剂完全相同,一种对解毒剂活性至关重要的氨基酸对杀伤功能也是必不可少的。通过对全球700多份拟南芥(a.thaliana)材料的调查,我们证实了该位点存在重要的结构重排和拷贝数变异。通过利用现有的从头基因组序列,我们提出了这种花粉杀手在拟南芥中出现的情景。此外,我们报告了杀伤基因型和敏感基因型在几个地方种群中的共同出现和行为,这是研究野生配子杀伤基因型进化的先决条件。这突出了拟南芥模型的潜力,不仅可以用于配子杀手的功能研究,还可以用于在互补的地理尺度上研究它们的进化轨迹。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana.

Gamete killers are genetic loci that distort segregation in the progeny of hybrids because the killer allele promotes the elimination of the gametes that carry the sensitive allele. They are widely distributed in eukaryotes and are important for understanding genome evolution and speciation. We had previously identified a pollen killer in hybrids between two distant natural accessions of Arabidopsis thaliana. This pollen killer involves three genetically linked genes, and we previously reported the identification of the gene encoding the antidote that protects pollen grains from the killer activity. In this study, we identified the two other genes of the pollen killer by using CRISPR-Cas9 induced mutants. These two genes are necessary for the killer activity that we demonstrated to be specific to pollen. The cellular localization of the pollen killer encoded proteins suggests that the pollen killer activity involves the mitochondria. Sequence analyses reveal predicted domains from the same families in the killer proteins. In addition, the C-terminal half of one of the killer proteins is identical to the antidote, and one amino acid, crucial for the antidote activity, is also essential for the killer function. Investigating more than 700 worldwide accessions of A. thaliana, we confirmed that the locus is subject to important structural rearrangements and copy number variation. By exploiting available de novo genomic sequences, we propose a scenario for the emergence of this pollen killer in A. thaliana. Furthermore, we report the co-occurrence and behavior of killer and sensitive genotypes in several local populations, a prerequisite for studying gamete killer evolution in the wild. This highlights the potential of the Arabidopsis model not only for functional studies of gamete killers but also for investigating their evolutionary trajectories at complementary geographical scales.

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来源期刊
PLoS Genetics
PLoS Genetics GENETICS & HEREDITY-
自引率
2.20%
发文量
438
期刊介绍: PLOS Genetics is run by an international Editorial Board, headed by the Editors-in-Chief, Greg Barsh (HudsonAlpha Institute of Biotechnology, and Stanford University School of Medicine) and Greg Copenhaver (The University of North Carolina at Chapel Hill). Articles published in PLOS Genetics are archived in PubMed Central and cited in PubMed.
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