[基于遗传多样性的黑骨肉鸡(Gallus Gallus domesticus)家系构建]。

Q3 Pharmacology, Toxicology and Pharmaceutics
Jiao Liu, Hai-Yun Gao, Yuan Yuan, Tian-Rui Liu, Hui Li, Tian Zhang
{"title":"[基于遗传多样性的黑骨肉鸡(Gallus Gallus domesticus)家系构建]。","authors":"Jiao Liu, Hai-Yun Gao, Yuan Yuan, Tian-Rui Liu, Hui Li, Tian Zhang","doi":"10.19540/j.cnki.cjcmm.20240830.101","DOIUrl":null,"url":null,"abstract":"<p><p>Black-bone silky fowls(Gallus gallus domesticus) have a long history of medicinal use, with the origin in Taihe county, Jiangxi province. The unclear family composition, inbreeding rate, and effective population size were inconducive to the resource conservation or breed improvement of black-bone silky fowls. A genome-wide analysis was performed to evaluate the genetic diversity of 80 black-bone silky fowls from random mating in three farms in 2021 in terms of minor allele frequency(MAF), expected heterozygosity(H_e), observed heterozygosity(H_o), effective population size(N_e), and runs of homozygosity(ROH). The results showed that a total of 9 641 411 SNPs were passed quality control from 80 black-bone silky fowls. The polymorphic marker ratio, expected heterozygosity, and observed heterozygosity in this population were 0.81, 0.34, and 0.33, respectively, and the average IBS genetic distance of black-bone silky fowls was 0.38±0.008 2. A total of 15 969 ROHs were identified, with the average length of 0.73 Mb, and most of the ROHs were 0~2 Mb. According to inbreeding ROHs, the average inbreeding coefficient was 0.150 9. The high-frequency ROH regions harbored 424 genes, including two genes, SLC13A4 and DDX6, associated with pigmentation. The results showed that the genetic relationship between individuals of black-bone silky fowls was far. The population had experienced a strong decline in size in recent decades, and the inbreeding degree was high. According to the results, an appropriate family construction scheme was proposed for resource conservation and breeding work of black-bone silky fowls.</p>","PeriodicalId":52437,"journal":{"name":"Zhongguo Zhongyao Zazhi","volume":"49 23","pages":"6281-6290"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"[Construction of black-bone silky fowl (Gallus gallus domesticus) families based on genetic diversity].\",\"authors\":\"Jiao Liu, Hai-Yun Gao, Yuan Yuan, Tian-Rui Liu, Hui Li, Tian Zhang\",\"doi\":\"10.19540/j.cnki.cjcmm.20240830.101\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Black-bone silky fowls(Gallus gallus domesticus) have a long history of medicinal use, with the origin in Taihe county, Jiangxi province. The unclear family composition, inbreeding rate, and effective population size were inconducive to the resource conservation or breed improvement of black-bone silky fowls. A genome-wide analysis was performed to evaluate the genetic diversity of 80 black-bone silky fowls from random mating in three farms in 2021 in terms of minor allele frequency(MAF), expected heterozygosity(H_e), observed heterozygosity(H_o), effective population size(N_e), and runs of homozygosity(ROH). The results showed that a total of 9 641 411 SNPs were passed quality control from 80 black-bone silky fowls. The polymorphic marker ratio, expected heterozygosity, and observed heterozygosity in this population were 0.81, 0.34, and 0.33, respectively, and the average IBS genetic distance of black-bone silky fowls was 0.38±0.008 2. A total of 15 969 ROHs were identified, with the average length of 0.73 Mb, and most of the ROHs were 0~2 Mb. According to inbreeding ROHs, the average inbreeding coefficient was 0.150 9. The high-frequency ROH regions harbored 424 genes, including two genes, SLC13A4 and DDX6, associated with pigmentation. The results showed that the genetic relationship between individuals of black-bone silky fowls was far. The population had experienced a strong decline in size in recent decades, and the inbreeding degree was high. According to the results, an appropriate family construction scheme was proposed for resource conservation and breeding work of black-bone silky fowls.</p>\",\"PeriodicalId\":52437,\"journal\":{\"name\":\"Zhongguo Zhongyao Zazhi\",\"volume\":\"49 23\",\"pages\":\"6281-6290\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Zhongguo Zhongyao Zazhi\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.19540/j.cnki.cjcmm.20240830.101\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zhongguo Zhongyao Zazhi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.19540/j.cnki.cjcmm.20240830.101","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
引用次数: 0

摘要

黑骨乌骨鸡药用历史悠久,原产于江西省泰和县。黑骨乌鸡的家系组成、近交率和有效种群数量不明确,不利于黑骨乌鸡的资源保护和品种改良。本研究从小等位基因频率(MAF)、预期杂合度(H_e)、观察杂合度(H_o)、有效种群数量(N_e)和同源杂合度(ROH)等方面对2021年3个农场随机交配的80只乌骨鸡的遗传多样性进行了全基因组分析。结果表明,80 只乌骨鸡中共有 9 641 411 个 SNPs 通过了质量控制。该群体的多态性标记比、预期杂合度和观察杂合度分别为0.81、0.34和0.33,黑骨乌骨鸡的平均IBS遗传距离为(0.38±0.008)2。共鉴定出15 969个ROH,平均长度为0.73 Mb,大部分ROH长度为0~2 Mb。根据近交ROHs,平均近交系数为0.150 9。高频ROH区域含有424个基因,其中包括与色素沉着有关的SLC13A4和DDX6两个基因。结果表明,黑骨乌骨鸡个体间的遗传关系较远。近几十年来,该种群的数量急剧下降,近亲繁殖程度较高。根据研究结果,为黑骨乌鸡的资源保护和育种工作提出了适当的家系构建方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Construction of black-bone silky fowl (Gallus gallus domesticus) families based on genetic diversity].

Black-bone silky fowls(Gallus gallus domesticus) have a long history of medicinal use, with the origin in Taihe county, Jiangxi province. The unclear family composition, inbreeding rate, and effective population size were inconducive to the resource conservation or breed improvement of black-bone silky fowls. A genome-wide analysis was performed to evaluate the genetic diversity of 80 black-bone silky fowls from random mating in three farms in 2021 in terms of minor allele frequency(MAF), expected heterozygosity(H_e), observed heterozygosity(H_o), effective population size(N_e), and runs of homozygosity(ROH). The results showed that a total of 9 641 411 SNPs were passed quality control from 80 black-bone silky fowls. The polymorphic marker ratio, expected heterozygosity, and observed heterozygosity in this population were 0.81, 0.34, and 0.33, respectively, and the average IBS genetic distance of black-bone silky fowls was 0.38±0.008 2. A total of 15 969 ROHs were identified, with the average length of 0.73 Mb, and most of the ROHs were 0~2 Mb. According to inbreeding ROHs, the average inbreeding coefficient was 0.150 9. The high-frequency ROH regions harbored 424 genes, including two genes, SLC13A4 and DDX6, associated with pigmentation. The results showed that the genetic relationship between individuals of black-bone silky fowls was far. The population had experienced a strong decline in size in recent decades, and the inbreeding degree was high. According to the results, an appropriate family construction scheme was proposed for resource conservation and breeding work of black-bone silky fowls.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Zhongguo Zhongyao Zazhi
Zhongguo Zhongyao Zazhi Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)
CiteScore
1.50
自引率
0.00%
发文量
581
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信