L F Minnullina, P S Misheeva, G I Mukhtarova, A M Mardanova
{"title":"尿尿素浓度对尿源摩氏摩根菌溶血活性和细胞毒性的影响。","authors":"L F Minnullina, P S Misheeva, G I Mukhtarova, A M Mardanova","doi":"10.1007/s10517-025-06312-2","DOIUrl":null,"url":null,"abstract":"<p><p>We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation. Our findings indicate a correlation between this activity and the expression of the hlyA gene. Bacterial culture fluid containing hemolysin caused the death of up to 40% of urothelial cells; cytotoxicity was more pronounced against OKP-GS kidney carcinoma cells compared to T-24 bladder carcinoma cells. Hence, in urine with higher urea concentrations, hemolytic activity of bacteria was observed at later stages of growth, and this culture fluid exhibited higher cytotoxicity.</p>","PeriodicalId":9331,"journal":{"name":"Bulletin of Experimental Biology and Medicine","volume":"178 2","pages":"227-232"},"PeriodicalIF":0.9000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Influence of Urinary Urea Concentration on Hemolytic Activity and Cytotoxicity of Uropathogenic Morganella morganii Strain.\",\"authors\":\"L F Minnullina, P S Misheeva, G I Mukhtarova, A M Mardanova\",\"doi\":\"10.1007/s10517-025-06312-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation. Our findings indicate a correlation between this activity and the expression of the hlyA gene. Bacterial culture fluid containing hemolysin caused the death of up to 40% of urothelial cells; cytotoxicity was more pronounced against OKP-GS kidney carcinoma cells compared to T-24 bladder carcinoma cells. Hence, in urine with higher urea concentrations, hemolytic activity of bacteria was observed at later stages of growth, and this culture fluid exhibited higher cytotoxicity.</p>\",\"PeriodicalId\":9331,\"journal\":{\"name\":\"Bulletin of Experimental Biology and Medicine\",\"volume\":\"178 2\",\"pages\":\"227-232\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bulletin of Experimental Biology and Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s10517-025-06312-2\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of Experimental Biology and Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10517-025-06312-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/7 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Influence of Urinary Urea Concentration on Hemolytic Activity and Cytotoxicity of Uropathogenic Morganella morganii Strain.
We studied the effect of urinary urea concentration on the hemolysin production and cytotoxicity of the uropathogenic Morganella morganii strain MM 190. The highest hemolytic activity of M. morganii cultivated in urine with low urea concentration (23 and 82 mmol/liter) was observed between 3rd and 4th hours of post-inoculation, while in urine with standard urea level (117 mmol/liter), the activity was observed at 5th hour of post-inoculation. Our findings indicate a correlation between this activity and the expression of the hlyA gene. Bacterial culture fluid containing hemolysin caused the death of up to 40% of urothelial cells; cytotoxicity was more pronounced against OKP-GS kidney carcinoma cells compared to T-24 bladder carcinoma cells. Hence, in urine with higher urea concentrations, hemolytic activity of bacteria was observed at later stages of growth, and this culture fluid exhibited higher cytotoxicity.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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