嗜热降解联合体对石油污染土壤生物强化的动态响应：碳氢化合物、微生物群落和功能基因

IF 12.2 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

Journal of Hazardous Materials Pub Date : 2025-01-14 DOI:10.1016/j.jhazmat.2025.137222

Qingling Wang, Jinyu Hou, Li Peng, Wuxing Liu, Yongming Luo

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引用次数: 0

摘要

生物强化为石油污染土壤的生物修复提供了一种有效的策略。然而，目前对高温条件下嗜热联合体降解石油烃的研究还很少。利用嗜热石油降解联合体HT建立了一个微观环境，研究了烃类降解、微生物群落和功能基因的响应。结果表明，高温联合处理显著提高了ph在140 d内的降解效果，其中对中质（C16-C21）和长链烷烃（C21-C40）的降解效果最为显著（87.1%）；HT在土壤中的定殖表现出滞后性，60天后HT产生的细菌属数量大幅增加。此外，LEfSe分析表明，HT治疗的生物标志物主要来自HT财团。此外，功能分析显示，正构烷烃降解相关基因（AlkB、P450、LadA）、烷烃利用调节基因（AraC、TetR、GntR）以及一些耐热基因在高温处理下显著增加。此外，网络分析显示，营养添加和外源联合诱导了不同的共发生模式，后者加强了细菌网络在高温下的相互作用和稳定性。本研究开创性地研究了外源嗜热联合体对石油降解、细菌群落、功能基因和生态相互作用的影响，并在嗜热条件下应用于石油修复。

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Dynamic Responses in Bioaugmentation of Petroleum-Contaminated Soils using Thermophilic Degrading Consortium HT: Hydrocarbons, Microbial Communities, and Functional Genes

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Dynamic Responses in Bioaugmentation of Petroleum-Contaminated Soils using Thermophilic Degrading Consortium HT: Hydrocarbons, Microbial Communities, and Functional Genes

Bioaugmentation offers an effective strategy for the bioremediation of petroleum-contaminated soils. However, little is known about petroleum hydrocarbons (PHs) degradation with thermophilic consortium application under high temperature. A microcosm was established to study hydrocarbons degradation, microbial communities and functional genes response using a thermophilic petroleum-degrading consortium HT. The results showed that the consortium HT significantly enhanced PHs degradation, particularly for medium (C16-C21) (87.1%) and long-chain alkanes (C21-C40) (67.2%) within 140 days under high temperature. Colonization of HT in the soil exhibited lagged characteristics, with a substantial increase in bacterial genera originated from the HT after 60 days. Additionally, LEfSe analysis indicated that the biomarkers of HT treatment were mainly from the HT consortium. Moreover, functional analysis revealed genes related to n-alkane degradation (AlkB, P450, LadA), alkane utilization regulator (AraC, TetR, GntR), as well as several thermotolerance genes were significantly increased in HT treatment. Additionally, network analysis demonstrated distinct co-occurrence patterns induced by nutrient addition and exogenous consortium, with the latter strengthening interactions and stability of bacterial networks under high temperature. This study represents pioneering investigation into the effects of exogenous thermophilic consortium on petroleum degradation, bacterial communities, functional genes and ecological interactions in application of petroleum remediation under thermophilic conditions.

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来源期刊

Journal of Hazardous Materials 工程技术-工程：环境

CiteScore

25.40

自引率

5.90%

发文量

3059

审稿时长

58 days

期刊介绍： The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.