小鼠外周感觉神经元膜蛋白酶体功能的研究方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-01-10 DOI:10.1016/j.xpro.2024.103552

Emily R Krueger, Taylor R Church, Anna Brennan, Fulya Türker, Eric Villalón Landeros

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引用次数: 0

摘要

神经元膜蛋白酶体（NMPs）在体感觉背根神经节（DRG）神经元的一个子集上表达，并影响机械和疼痛敏感性。在此，我们提出了一种研究小鼠外周感觉神经元NMP功能的方案。我们描述了获取和培养初级DRG神经元的步骤。然后，我们详细介绍了生化和抗体喂养方法来分析NMP的表达和定位。最后，我们采用Ca2+成像技术来研究DRG神经元的NMP功能。有关本协议使用和执行的完整细节，请参阅Villalón Landeros et al.1。

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Protocol to study neuronal membrane proteasome function in mouse peripheral sensory neurons.

Neuronal membrane proteasomes (NMPs) are expressed on a subset of somatosensory dorsal root ganglion (DRG) neurons and influence mechanical and pain sensitivity. Here, we present a protocol for studying NMP function in mouse peripheral sensory neurons. We describe steps for procuring and culturing primary DRG neurons. We then detail biochemical and antibody feeding approaches to analyze NMP expression and localization. Finally, we include Ca²⁺ imaging techniques for investigating NMP function in DRG neurons. For complete details on the use and execution of this protocol, please refer to Villalón Landeros et al.¹.

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