用于测量人体细胞中葡萄糖和乳酸代谢变化的活细胞代谢分析仪协议。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2025-01-10 DOI:10.1016/j.xpro.2024.103518

Kenji Miki, Mikako Yagi, Ko Igami, Hiroki Kittaka, Akito Tani, Natsuki Horiuchi, Satoshi Fukumoto, Koji Yoshimoto, Takeshi Uchiumi

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引用次数: 0

摘要

了解与糖酵解依赖性相关的代谢条件对于开发癌症和再生医学的新疗法至关重要。本方案详细介绍了使用活细胞代谢分析仪（LiCellMo）测量培养的人类细胞中葡萄糖消耗和乳酸生成的连续变化的方法。LiCellMo提供连续代谢变化的实时数据，改进了各种情况下这些过程的测量，包括癌症和再生治疗。

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Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells.

Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

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