通过CRISPR/(WAe009-A-2K) Cas9编辑生成PDK-1敲除的人胚胎干细胞系

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2024-12-28 DOI:10.1016/j.scr.2024.103642

Amina Saleem , Mingyu Wei , Muhammad Khawar Abbas , Siyao Zhang , Jiaqi Fan , Yang Xian , Hongfeng Jiang

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引用次数: 0

摘要

丙酮酸脱氢酶激酶1 （Pyruvate Dehydrogenase Kinase1, PDK1）属于激酶家族，调控多种代谢过程。PDK1是心衰的易感性位点，可导致心室壁变薄、心房和心室增大。我们利用episomal载体CRISPR/Cas9系统成功开发了PDK1敲除（PDK1-/-）人胚胎干细胞（hESC）系，探索PDK在人类心脏发育中的作用。这种PDK1-KO hESC细胞系维持干细胞样形态、多能性和正常核型，并能在体内分化为所有三种胚层。该细胞系将为未来研究PDK1在心脏发育中的作用提供有价值的工具。

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Generation of a PDK-1 knockout human embryonic stem cell line by CRISPR/(WAe009-A-2K) Cas9 editing

Pyruvate Dehydrogenase Kinase1 (PDK1) belongs to the family of kinases, regulates diverse metabolic processes. PDK1 is a susceptibility locus for heart failure via thinning of ventricle walls, and enlarged atria and ventricles. We successfully developed a PDK1 knockout (PDK1⁻/⁻) human embryonic stem cell (hESC) line using an episomal vector-based CRISPR/Cas9 system explore the role of PDK in human heart development. This PDK1-KO hESC line-maintained stem cell-like morphology, pluripotency, and normal karyotype and can differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of PDK1 in heart development.

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.