在捷克共和国,家鼠(小家鼠)广泛的抗凝血药耐药性与Tyr139Phe突变有关。

IF 3.9 2区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Marcela Frankova, Zuzana Starostova, Radek Aulicky, Vaclav Stejskal
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引用次数: 0

摘要

尽管抗凝血灭鼠剂广泛应用于饵料中以控制共生鼠害,但由于其继发性中毒和抗性增加,其应用存在问题。与对西欧家鼠(家鼠)的研究相比,对东欧家鼠(家鼠)的抗性研究很少,东欧家鼠的分布边界在捷克共和国西部。本研究最新分析了捷克农场和粮库中m.m. musus田间种群的VKORC1基因,并鉴定出一个非同义突变Tyr139Phe。这种突变在整个捷克共和国都很常见,86个样本中有80.2%存在这种突变。此外,所有个体都表现出一种具有三个同义突变的基因型,这是m.m.肌肉亚种特有的。Tyr139Phe突变的功能性(死亡-生存)反应在使用溴代酮为基础的诱饵的实验室选择喂养试验中得到验证,其中所有耐药纯合子个体存活,而所有易感小鼠死亡,平均存活时间为6.9天。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic.

Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic.

Widespread anticoagulant resistance in house mice (Mus musculus musculus) linked to the Tyr139Phe mutation in the Czech Republic.

Despite the widespread use of anticoagulant rodenticides in baits for controlling commensal rodent pests, their application is problematic due to secondary intoxication and increasing resistance. In contrast to studies on Western European house mice (Mus musculus domesticus), few resistance studies have focused on Eastern European house mice (M. musculus musculus), which have a western distribution boundary in the Czech Republic. This study newly analysed the VKORC1 gene in M. m. musculus field populations from Czech farms and grain stores and identified a nonsynonymous mutation Tyr139Phe. This mutation was common throughout the Czech Republic and was present in 80.2% of the 86 individuals sampled. Additionally, all individuals exhibited a genotype with three synonymous mutations specific to the subspecies M. m. musculus. The functional (mortality-survival) response of the Tyr139Phe mutation was validated in a laboratory choice feeding test using bromadiolone-based bait, where all resistant homozygous individuals survived, while all susceptible mice died, with a mean survival of 6.9 days.

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来源期刊
Scientific Reports
Scientific Reports Natural Science Disciplines-
CiteScore
7.50
自引率
4.30%
发文量
19567
审稿时长
3.9 months
期刊介绍: We publish original research from all areas of the natural sciences, psychology, medicine and engineering. You can learn more about what we publish by browsing our specific scientific subject areas below or explore Scientific Reports by browsing all articles and collections. Scientific Reports has a 2-year impact factor: 4.380 (2021), and is the 6th most-cited journal in the world, with more than 540,000 citations in 2020 (Clarivate Analytics, 2021). •Engineering Engineering covers all aspects of engineering, technology, and applied science. It plays a crucial role in the development of technologies to address some of the world''s biggest challenges, helping to save lives and improve the way we live. •Physical sciences Physical sciences are those academic disciplines that aim to uncover the underlying laws of nature — often written in the language of mathematics. It is a collective term for areas of study including astronomy, chemistry, materials science and physics. •Earth and environmental sciences Earth and environmental sciences cover all aspects of Earth and planetary science and broadly encompass solid Earth processes, surface and atmospheric dynamics, Earth system history, climate and climate change, marine and freshwater systems, and ecology. It also considers the interactions between humans and these systems. •Biological sciences Biological sciences encompass all the divisions of natural sciences examining various aspects of vital processes. The concept includes anatomy, physiology, cell biology, biochemistry and biophysics, and covers all organisms from microorganisms, animals to plants. •Health sciences The health sciences study health, disease and healthcare. This field of study aims to develop knowledge, interventions and technology for use in healthcare to improve the treatment of patients.
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