治疗性超声调节骨关节炎软骨细胞的细胞增殖和促炎细胞因子水平

IF 5.3
Ahmet Çagdas Yilmaz, Hasan Toktas, Sefa Celik, Serkan Sen
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引用次数: 0

摘要

骨关节炎(OA)的发生和发展被认为与炎症有关。本研究旨在探讨治疗性超声(US)在连续和脉冲模式下对人骨关节炎软骨细胞增殖和促炎细胞因子水平的影响。人骨关节炎软骨细胞(HC-OA 402o -05a)在适当的培养基中增殖,然后接种到培养板中。将实验板分组,以0.1 W/cm2和1 MHz的频率连续、脉冲和对照暴露于水下,每天10分钟,持续10天。MTT法测定细胞活力/增殖,ELISA法测定总蛋白,测定每蛋白细胞因子水平。用显微镜分析对细胞进行拍照。与对照组相比,连续和脉冲美国组的生存能力都有显著提高。连续组和脉冲组之间IL-1β、TNF-α和IL-6水平无显著差异。与对照组相比,两组细胞因子均显著减少。对于IL-17和IL-32水平,两组细胞因子水平均低于对照组,但结果不显著。0.1 W/cm2和1 MHz的水下超声刺激骨关节炎软骨细胞增殖,降低促炎细胞因子水平。本研究广泛关注促炎IL水平,有意义的结果可能启发未来的体内/体外研究。虽然使体外数据适应体内条件存在挑战,但我们的结果可以指导未来的体内研究和临床应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Therapeutic Ultrasound Modulates Cell Proliferation and Proinflammatory Cytokine Levels in Osteoarthritic Chondrocytes

Therapeutic Ultrasound Modulates Cell Proliferation and Proinflammatory Cytokine Levels in Osteoarthritic Chondrocytes

The development and progression of osteoarthritis (OA) are believed to involve inflammation. This study aimed to investigate the effects of applying therapeutic ultrasound (US) to human osteoarthritic chondrocytes in continuous and pulsed modes on cell proliferation and proinflammatory cytokine levels. Human osteoarthritic chondrocytes (HC-OA 402OA-05a) were proliferated in appropriate media and then seeded into culture plates. The plates were grouped and exposed to underwater continuous, pulsed and control US at 0.1 W/cm2 and 1 MHz for 10 min daily for 10 days. Cell viability/proliferation was assessed using the MTT assay, total protein was measured by ELISA and cytokine levels per protein were determined. Cells were photographed using microscopic analysis. Both continuous and pulsed US groups showed a significant increase in viability compared to the control group. No significant difference was found between the continuous and pulsed US groups for IL-1β, TNF-α and IL-6 levels. Both groups showed significant cytokine reduction compared to the control group. For IL-17 and IL-32 levels, both US groups had reduced cytokine levels compared to the control group, but the results were not significant. Underwater US at 0.1 W/cm2 and 1 MHz stimulated cell proliferation and reduced proinflammatory cytokine levels in osteoarthritic chondrocyte cell cultures. This study extensively focused on proinflammatory IL levels, and the meaningful results may inspire future in vivo/in vitro studies. While adapting in vitro data to in vivo conditions poses challenges, our results could guide future in vivo studies and clinical applications.

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来源期刊
CiteScore
11.50
自引率
0.00%
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期刊介绍: The Journal of Cellular and Molecular Medicine serves as a bridge between physiology and cellular medicine, as well as molecular biology and molecular therapeutics. With a 20-year history, the journal adopts an interdisciplinary approach to showcase innovative discoveries. It publishes research aimed at advancing the collective understanding of the cellular and molecular mechanisms underlying diseases. The journal emphasizes translational studies that translate this knowledge into therapeutic strategies. Being fully open access, the journal is accessible to all readers.
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