用氧化锡/氮化石墨碳修饰的碳纤维作为超灵敏黄曲霉毒素 M1 检测的电化学间接竞争免疫传感器。

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-01-09 DOI:10.1016/j.bioelechem.2025.108898

Iram Naz , Muhammad Nasir , Mian Hasnain Nawaz , Silvana Andreescu , Akhtar Hayat , Farhat Jubeen

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引用次数: 0

摘要

随着纳米技术的发展，开发用于传感技术的多功能纳米材料的重要性日益增加。在这项研究中，我们描述了将新型纳米探针电活性材料引入电化学免疫传感器的结构中。在电化学免疫传感器的基础上，合成了功能化氧化锡/石墨氮化碳纳米复合材料（fSnO2/g-C3N4），并结合分析特异性抗黄曲霉毒素M1单克隆抗体（AFM1-ab）形成电活性纳米探针（fSnO2/g-C3N4/AFM1-ab）。首先，在碳纤维（CF）表面电氧化黄曲霉毒素M1 （AFM1）偶联牛血清白蛋白（BSA-AFM1），然后添加纳米探针。通过傅里叶变换红外光谱（FT-IR）、拉曼光谱、x射线衍射（XRD）、热重分析（TGA）和动态光散射（DLS）等表征技术证实了纳米复合材料的形成。通过场发射扫描电镜（FE-SEM）、光学显微镜图像、循环伏安法（CV）、电化学阻抗谱（EIS）和差分脉冲伏安法（DPV）对免疫传感器的制备进行了表征。该免疫传感器对AFM1具有良好的重现性、选择性、特异性和敏感性（LOD为0.03 ng mL-1）。在峰值之后，该免疫传感器对真实样品（如牛奶）产生了良好的回收率，范围为94- 96%。这种创新的免疫传感方法的广泛应用可以极大地受益于各种分析物的复杂传感方法的发展。

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A carbon fiber modified with tin oxide/graphitic carbon nitride as an electrochemical indirect competitive immuno-sensor for ultrasensitive aflatoxin M1 detection

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A carbon fiber modified with tin oxide/graphitic carbon nitride as an electrochemical indirect competitive immuno-sensor for ultrasensitive aflatoxin M1 detection

The importance of developing multifunctional nanomaterials for sensing technologies is increasing with the arrival of nanotechnology. In this study, we describe the introduction of novel nanoprobe electro-active material into the architecture of an electrochemical immuno-sensor. Based on the electrochemical immuno-sensor, functionalized tin oxide/graphitic carbon nitride nanocomposite (fSnO₂/g-C₃N₄) was synthesized and then analyte specific anti-aflatoxin M₁ monoclonal antibody (AFM₁-ab) combined to form an electro-active nanoprobe (fSnO₂/g-C₃N₄/AFM₁-ab). First, aflatoxin M₁ (AFM₁) conjugated bovine serum albumin (BSA-AFM₁) was electro-oxidized on the surface of carbon fiber (CF) followed by the consequent addition of nanoprobe. The formation of nanocomposite was substantiated through various characterization techniques, Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, X-ray diffraction (XRD), Thermogravimetric analysis (TGA) and Dynamic light scattering (DLS). Immuno-sensor fabrication was characterized via Field emission scanning electron microscopy (FE-SEM), optical microscope images, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). This immuno-sensor demonstrated good reproducibility, selectivity, specificity and sensitivity for AFM₁ (LOD of 0.03 ng mL⁻¹). Following spiking, this immuno-sensor produced good recovery values in the range of 94–96 % against real sample, such as milk. The development of sophisticated sensing methods for a range of analytes can greatly benefit from the widespread application of this innovative immuno-sensing approach.

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.