{"title":"intI1作为指示基因在污水处理厂中确保细胞外抗菌素耐药基因去除效率的适用性","authors":"Rongxuan Wang, Mardalisa, Hiroe Hara-Yamamura, Norihisa Matsuura, Ryo Honda","doi":"10.1016/j.biortech.2025.132047","DOIUrl":null,"url":null,"abstract":"<p><p>Mitigating the release of extracellular antimicrobial resistance genes (exARGs) from wastewater treatment plants (WWTPs) is crucial for preventing the spread of antimicrobial resistance from human domains into the environment. This study aimed to evaluate the applicability of intI1 as a performance indicator for securing the removal of exARGs at WWTPs. We investigated the reduction of exARGs and intI1 in a full-scale WWTP, where identical wastewater was treated using conventional activated sludge (CAS) and membrane bioreactor (MBR) systems. The log reduction values (LRVs) for exARGs were lower than those for intracellular ARGs (iARGs) across all ARG species and treatment systems. LRVs for exARGs were consistently higher in the MBR than in the CAS. The intI1 exhibited lower LRVs compared to most exARGs, ensuring a minimum LRV of exARG in both CAS and MBR systems. Consequently, intI1 is an effective indicator gene for securing the removal of exARGs.</p>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":" ","pages":"132047"},"PeriodicalIF":9.7000,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Applicability of intI1 as an indicator gene for securing the removal efficiency of extracellular antimicrobial resistance genes in full-scale wastewater treatment plants.\",\"authors\":\"Rongxuan Wang, Mardalisa, Hiroe Hara-Yamamura, Norihisa Matsuura, Ryo Honda\",\"doi\":\"10.1016/j.biortech.2025.132047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mitigating the release of extracellular antimicrobial resistance genes (exARGs) from wastewater treatment plants (WWTPs) is crucial for preventing the spread of antimicrobial resistance from human domains into the environment. This study aimed to evaluate the applicability of intI1 as a performance indicator for securing the removal of exARGs at WWTPs. We investigated the reduction of exARGs and intI1 in a full-scale WWTP, where identical wastewater was treated using conventional activated sludge (CAS) and membrane bioreactor (MBR) systems. The log reduction values (LRVs) for exARGs were lower than those for intracellular ARGs (iARGs) across all ARG species and treatment systems. LRVs for exARGs were consistently higher in the MBR than in the CAS. The intI1 exhibited lower LRVs compared to most exARGs, ensuring a minimum LRV of exARG in both CAS and MBR systems. Consequently, intI1 is an effective indicator gene for securing the removal of exARGs.</p>\",\"PeriodicalId\":258,\"journal\":{\"name\":\"Bioresource Technology\",\"volume\":\" \",\"pages\":\"132047\"},\"PeriodicalIF\":9.7000,\"publicationDate\":\"2025-01-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1016/j.biortech.2025.132047\",\"RegionNum\":1,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.biortech.2025.132047","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
Applicability of intI1 as an indicator gene for securing the removal efficiency of extracellular antimicrobial resistance genes in full-scale wastewater treatment plants.
Mitigating the release of extracellular antimicrobial resistance genes (exARGs) from wastewater treatment plants (WWTPs) is crucial for preventing the spread of antimicrobial resistance from human domains into the environment. This study aimed to evaluate the applicability of intI1 as a performance indicator for securing the removal of exARGs at WWTPs. We investigated the reduction of exARGs and intI1 in a full-scale WWTP, where identical wastewater was treated using conventional activated sludge (CAS) and membrane bioreactor (MBR) systems. The log reduction values (LRVs) for exARGs were lower than those for intracellular ARGs (iARGs) across all ARG species and treatment systems. LRVs for exARGs were consistently higher in the MBR than in the CAS. The intI1 exhibited lower LRVs compared to most exARGs, ensuring a minimum LRV of exARG in both CAS and MBR systems. Consequently, intI1 is an effective indicator gene for securing the removal of exARGs.
期刊介绍：
Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies.
Topics include:
• Biofuels: liquid and gaseous biofuels production, modeling and economics
• Bioprocesses and bioproducts: biocatalysis and fermentations
• Biomass and feedstocks utilization: bioconversion of agro-industrial residues
• Environmental protection: biological waste treatment
• Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
