同位素法放射性合成[18F]-氟马西尼。

IF 0.4 Q4 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
Indian Journal of Nuclear Medicine Pub Date : 2024-07-01 Epub Date: 2024-11-18 DOI:10.4103/ijnm.ijnm_82_24
Riptee Thakur, Aishwarya Kumar, Raman Kumar Joshi, Pardeep Kumar
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引用次数: 0

摘要

背景:氟-18 (18F)氟马西尼(FMZ)已由多种前体合成,并探索其在γ -氨基丁酸-a受体成像中的作用。目的与目的:主要目的是用同位素取代法合成(18F) FMZ。材料和方法:在模组中加入约18±2 GBq,干燥,用3.0 mg FMZ前体在不同温度(110°C -160°C)下10-30分钟进行标准化放射性标记。最后用20%乙醇(在磷酸盐缓冲液中)洗脱。最终产物通过高效液相色谱(HPLC)进行了表征。结果:筒式纯化的放射标记效率为16±4% (n = 10),放射化学纯度为96.5±1.8%;高效液相色谱纯化的收率为10±4% (n = 5),放射化学纯度为97.3±1.4%。比活性为120±20 GBq/μmol。结论:(18F) FMZ采用同位素法成功合成,可作为一种较便宜的合成方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Radiosynthesis of [18F]-flumazenil Using an Isotopic Approach.

Background: Fluorine-18 (18F) flumazenil (FMZ) has been synthesized using various precursors, and its role has been explored in imaging Gamma-aminobutyric acid-A receptors.

Aim and objective: The main objective was to synthesize (18F) FMZ using isotopic substitution.

Materials and methods: Around 18 ± 2 GBq was added to the module, dried, and radiolabeling was standardized with 3.0 mg of the FMZ precursor at various temperatures (110°C -160°C) for 10-30 min. The product was finally eluted with 20% ethanol (in phosphate buffer). The final product was characterized by high-performance liquid chromatography (HPLC). The stability was evaluated in water, saline, and phosphate-buffered saline for 4 h.

Results: The radiolabelling efficiency of cartridge-based purification was 16 ± 4% (n = 10) with a radiochemical purity of 96.5 ± 1.8%, whereas in HPLC-based purification, the yield was 10 ± 4% (n = 5) with a radiochemical purity of 97.3 ± 1.4%. The specific activity was 120 ± 20 GBq/μmol.

Conclusions: (18F) FMZ was successfully synthesized using an isotopic approach and could be used as an alternative cheaper option for the synthesis.

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来源期刊
Indian Journal of Nuclear Medicine
Indian Journal of Nuclear Medicine RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
0.70
自引率
0.00%
发文量
46
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