利用CRISPR/Cas9技术产生KDM5D基因敲除的人胚胎干细胞系

IF 0.8 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-01-03 DOI:10.1016/j.scr.2025.103651

Ouhui Li , Yuting Zhen , Chao Sun , Yanlin Ma , Qi Li , Luan Wen

{"title":"利用CRISPR/Cas9技术产生KDM5D基因敲除的人胚胎干细胞系","authors":"Ouhui Li , Yuting Zhen , Chao Sun , Yanlin Ma , Qi Li , Luan Wen","doi":"10.1016/j.scr.2025.103651","DOIUrl":null,"url":null,"abstract":"<div><div><em>KDM5D</em> is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a <em>KDM5D</em> knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D’s function in spermatogenesis and its influence on sex differences in various diseases.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"83 ","pages":"Article 103651"},"PeriodicalIF":0.8000,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Generation of a KDM5D knockout human embryonic stem cell line with CRISPR/Cas9 technology\",\"authors\":\"Ouhui Li , Yuting Zhen , Chao Sun , Yanlin Ma , Qi Li , Luan Wen\",\"doi\":\"10.1016/j.scr.2025.103651\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div><em>KDM5D</em> is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a <em>KDM5D</em> knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D’s function in spermatogenesis and its influence on sex differences in various diseases.</div></div>\",\"PeriodicalId\":21843,\"journal\":{\"name\":\"Stem cell research\",\"volume\":\"83 \",\"pages\":\"Article 103651\"},\"PeriodicalIF\":0.8000,\"publicationDate\":\"2025-01-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Stem cell research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1873506125000017\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Stem cell research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1873506125000017","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

KDM5D是一种与生精失败和结肠癌进展中的性别相关差异有关的基因，尽管其在精子发生中的作用尚不清楚。我们利用CRISPR/Cas9技术成功生成了KDM5D基因敲除的人胚胎干细胞。该敲除细胞系为研究KDM5D在精子发生中的功能及其对各种疾病性别差异的影响提供了有价值的模型。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Generation of a KDM5D knockout human embryonic stem cell line with CRISPR/Cas9 technology

KDM5D is a gene implicated in spermatogenic failure and sex-related differences in colon cancer progression, though its role in spermatogenesis remains unclear. We successfully generated a KDM5D knockout human embryonic stem cells using CRISPR/Cas9 technology. This knockout cell line provides a valuable model for studying KDM5D’s function in spermatogenesis and its influence on sex differences in various diseases.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.