认知障碍与海马铁超载的关系：大鼠模型的定量易感性图谱研究。

IF 4.7 2区医学 Q1 NEUROIMAGING

NeuroImage Pub Date : 2025-02-01 DOI:10.1016/j.neuroimage.2025.121006

Xi Deng , Meiru Bu , Jiali Liang , Yihao Sun , Liyan Li , Heishu Zheng , Zisan Zeng , Muliang Jiang , Bihong T. Chen

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引用次数: 0

摘要

背景：本研究的目的是建立铁超载大鼠模型来模拟地中海贫血患者铁水平升高，并探讨海马铁沉积与认知之间的潜在关联。方法：采用两组铁超载大鼠和一组对照大鼠进行研究。采用Morris水迷宫（Morris water maze， MWM）测试空间参考记忆，以逃避潜伏期和穿越Morris水迷宫平台的次数为指标。通过定量敏感性制图（QSM）评估海马组织的磁化率值，作为铁沉积的测量指标，并与空间参考记忆性能相关。采用二氨基联苯胺（DAB）增强Perl’s Prussian blue （PPB）染色法测定大鼠海马组织切片铁含量。结果：铁超载组（H组和L组）海马磁化率值高于对照组（d组），铁超载组的MWM逃逸潜伏期比对照组长，MWM平台穿越次数减少。H组和l组小鼠平均逃避潜伏期与海马平均磁化率值呈正相关，穿越平台次数与海马平均磁化率值呈负相关，穿越平台次数与潜伏逃避时间呈负相关。这个模拟地中海贫血铁超载的大鼠模型显示海马铁超载与空间参考记忆障碍有关。QSM可用于量化体内脑铁超载，强调其在评估地中海贫血患者认知功能障碍方面的潜在临床应用。

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Relationship between cognitive impairment and hippocampal iron overload: A quantitative susceptibility mapping study of a rat model

Background

The aim of this study was to establish an iron overload rat model to simulate the elevated iron levels in patients with thalassemia and to investigate the potential association between hippocampal iron deposition and cognition.

Methods

Two groups of iron overloaded rats and one group of control rats were used for this study. The Morris water maze (MWM) was used to test spatial reference memory indicated by escape latency time and number of MWM platform crossings. The magnetic susceptibility value of the hippocampal tissue, a measure of iron deposition, was assessed by quantitative susceptibility mapping (QSM) and was correlated with spatial reference memory performance. The iron content in hippocampal tissue sections of the rats were assessed using diaminobenzidine (DAB)-enhanced Perl's Prussian blue (PPB) staining.

Results

The rat groups with iron overload including the Group H and Group L had higher hippocampal magnetic susceptibility values than the control rat group, i.e., Group D. In addition, the iron overloaded groups had longer MWM escape latency than the control group, and reduced number of MWM platform crossings. There was a positive correlation between the mean escape latency and the mean hippocampal magnetic susceptibility value, a negative correlation between the number of platform crossings and the mean hippocampal magnetic susceptibility value, and a negative correlation between the number of platform crossings and the latent escape time in Group H and Group L.

Conclusion

This rat model simulating iron overload in thalassemia showed hippocampal iron overload being associated with impairment of spatial reference memory. QSM could be used to quantify brain iron overload in vivo, highlighting its potential clinical application for assessing cognitive impairment in patients with thalassemia.

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来源期刊

NeuroImage 医学-核医学

CiteScore

11.30

自引率

10.50%

发文量

809

审稿时长

63 days

期刊介绍： NeuroImage, a Journal of Brain Function provides a vehicle for communicating important advances in acquiring, analyzing, and modelling neuroimaging data and in applying these techniques to the study of structure-function and brain-behavior relationships. Though the emphasis is on the macroscopic level of human brain organization, meso-and microscopic neuroimaging across all species will be considered if informative for understanding the aforementioned relationships.