具有肢芽间充质和顶端外胚层脊细胞报告基因的转基因鸡系的产生。

IF 2.5 3区生物学 Q2 DEVELOPMENTAL BIOLOGY

Developmental biology Pub Date : 2025-01-08 DOI:10.1016/j.ydbio.2025.01.003

Yuji Atsuta , Yi-Chen Chen , Yuna Hattori , Tatsuya Takemoto , Daisuke Saito

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引用次数: 0

摘要

细胞类型特异性报告基因转基因鸡系是发育生物学的宝贵工具，可以可视化活胚胎中目标细胞类型的动态和分化状态。在这里，我们报道了一种新的转基因鸡系的建立，在胚胎中，肢体间充质细胞和顶端外胚层脊（AER）细胞被不同的荧光蛋白标记。产生报告系的过程包括使用组织特异性启动子、Tol2转座子介导的基因组整合和原始生殖细胞的克隆培养系统。利用转基因鸡可以更详细地表征肢体间充质细胞和AER细胞。因此，该报道鸡系将成为推进脊椎动物肢体发育研究的有力工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Generation of a transgenic chicken line with reporters for limb bud mesenchyme and apical ectodermal ridge cells

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Generation of a transgenic chicken line with reporters for limb bud mesenchyme and apical ectodermal ridge cells

Cell type-specific reporter transgenic chicken lines are invaluable tools in developmental biology, allowing the visualization of dynamics and differentiation states of target cell types in living embryos. Here, we report the establishment of a new transgenic chicken line in which limb mesenchyme and apical ectodermal ridge (AER) cells are labeled with different fluorescent proteins in the embryos. The processes for generating the reporter line involved using tissue-specific promoters, the Tol2 transposon-mediated genomic integration, and clonal culture system of primordial germ cells. Employing the transgenic chickens would facilitate the detailed characterization of limb mesenchyme and AER cells. Thus, this reporter chicken line will be a powerful tool for advancing the study of vertebrate limb development.

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来源期刊

Developmental biology 生物-发育生物学

CiteScore

5.30

自引率

3.70%

发文量

182

审稿时长

1.5 months

期刊介绍： Developmental Biology (DB) publishes original research on mechanisms of development, differentiation, and growth in animals and plants at the molecular, cellular, genetic and evolutionary levels. Areas of particular emphasis include transcriptional control mechanisms, embryonic patterning, cell-cell interactions, growth factors and signal transduction, and regulatory hierarchies in developing plants and animals.