自来水样品中奈格里氏菌和福氏奈格里氏菌的检测与鉴定。

IF 2.1 4区 医学 Q3 GENETICS & HEREDITY
Muhammad Aurongzeb, Syeda Zehratul Fatima, Syed Ikhlaq Hussain, Yasmeen Rashid, Tariq Aziz, Majid Alhomrani, Walaa F Alsanie, Abdulhakeem S Alamri
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引用次数: 0

摘要

福氏奈格里菌是原发性阿米巴脑膜炎脑炎(PAM)的病原体,常见于温暖的淡水环境中,可在游泳或洗澡等活动中通过鼻道进入大脑。PAM死亡率高,令人担忧其对全球健康的影响。在巴基斯坦,特别是在卡拉奇,已经报告了大量病例,这些病例通常没有娱乐用水接触史,但经常使用自来水进行清洗。本研究利用ITS-引物和naegl引物对卡拉奇市自来水样品的理化参数、总大肠菌群和粪便大肠菌群丰度进行了分析,并对福氏奈格里菌和其他奈格里菌进行了PCR检测。几乎所有的样品都显示出高温、低氯含量和大量大肠菌群的存在。39份样品中有11份检出福氏奈格里菌和其他奈格里菌。序列分析在卡拉奇市Golimar和Lyari地区的自来水中鉴定出福氏奈格里氏菌,而其他9个样本显示出不同的奈格里氏菌种。该研究表明,高温、氯化不足和大肠菌群的存在可能为福氏乳杆菌的生长创造有利条件。然而,这些因素并非Golimar和Lyari地区所独有,这表明其他环境或基础设施因素(本研究未详细说明)可能导致福氏奈瑟氏菌在该特定地点的存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Detection and identification of Naegleria species along with Naegleria fowleri in the tap water samples.

Naegleria fowleri, the causative agent of Primary Amoebic Meningoencephalitis (PAM), is commonly found in warm freshwater environments and can enter the brain through nasal passages during activities like swimming or ablution. PAM has a high fatality rate, raising concerns about its global health impact. In Pakistan, particularly in Karachi, a significant number of cases have been reported, often with no history of recreational water exposure, but with regular ablution using tap water. This study analyzed the physicochemical parameters, abundance of total and fecal coliforms, and detected N. fowleri and other Naegleria species in tap water samples from Karachi using PCR with ITS- and Naegl-primers. Almost all samples exhibited high temperatures, low chlorine levels, and a high presence of coliforms. N. fowleri and other Naegleria species were detected in 11 out of 39 samples. Sequence analysis identified N. fowleri in tap water from the Golimar and Lyari areas of Karachi, while the other nine samples revealed different Naegleria species. This study suggests that the combination of high temperatures, insufficient chlorination, and the presence of coliforms may create favorable conditions for N. fowleri growth. However, these factors are not exclusive to the Golimar and Lyari areas, indicating that other environmental or infrastructural factors, not detailed in this study, may have contributed to the presence of N. fowleri in that specific location.

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来源期刊
BMC Medical Genomics
BMC Medical Genomics 医学-遗传学
CiteScore
3.90
自引率
0.00%
发文量
243
审稿时长
3.5 months
期刊介绍: BMC Medical Genomics is an open access journal publishing original peer-reviewed research articles in all aspects of functional genomics, genome structure, genome-scale population genetics, epigenomics, proteomics, systems analysis, and pharmacogenomics in relation to human health and disease.
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