MicroED在结构生物学和基于结构的药物发现中的应用。

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. General subjects Pub Date : 2025-01-04 DOI:10.1016/j.bbagen.2025.130758

Salma Mirza , Malik Shoaib Ahmad

{"title":"MicroED在结构生物学和基于结构的药物发现中的应用。","authors":"Salma Mirza , Malik Shoaib Ahmad","doi":"10.1016/j.bbagen.2025.130758","DOIUrl":null,"url":null,"abstract":"<div><div>Microcrystal electron diffraction (MicroED) is an emerging method for the structure determination of proteins and peptides, enzyme-inhibitor complexes. Several structures of biomolecules, including lysozyme, proteinase K, adenosine receptor A2A, insulin, xylanase, thermolysin, DNA, and Granulovirus occlusion bodies, have been successfully determined through MicroED. As MicroED uses very small crystals for structure determination, therefore, it has several advantages over conventional X-ray diffraction methods. In this review article, we discussed the most recent developments in the field of MicroED and its applications for the structural determination of different types of peptides, proteins, enzymes, DNA, and enzyme-inhibitor-complexed structures.</div></div>","PeriodicalId":8800,"journal":{"name":"Biochimica et biophysica acta. General subjects","volume":"1869 3","pages":"Article 130758"},"PeriodicalIF":2.8000,"publicationDate":"2025-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Applications of MicroED in structural biology and structure-based drug discovery\",\"authors\":\"Salma Mirza , Malik Shoaib Ahmad\",\"doi\":\"10.1016/j.bbagen.2025.130758\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Microcrystal electron diffraction (MicroED) is an emerging method for the structure determination of proteins and peptides, enzyme-inhibitor complexes. Several structures of biomolecules, including lysozyme, proteinase K, adenosine receptor A2A, insulin, xylanase, thermolysin, DNA, and Granulovirus occlusion bodies, have been successfully determined through MicroED. As MicroED uses very small crystals for structure determination, therefore, it has several advantages over conventional X-ray diffraction methods. In this review article, we discussed the most recent developments in the field of MicroED and its applications for the structural determination of different types of peptides, proteins, enzymes, DNA, and enzyme-inhibitor-complexed structures.</div></div>\",\"PeriodicalId\":8800,\"journal\":{\"name\":\"Biochimica et biophysica acta. General subjects\",\"volume\":\"1869 3\",\"pages\":\"Article 130758\"},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2025-01-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biochimica et biophysica acta. General subjects\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0304416525000030\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta. General subjects","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0304416525000030","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

微晶电子衍射（MicroED）是测定蛋白质和多肽、酶抑制剂复合物结构的一种新兴方法。一些生物分子的结构，包括溶菌酶、蛋白酶K、腺苷受体A2A、胰岛素、木聚糖酶、热溶酶、DNA和颗粒病毒阻断体，已经通过MicroED成功测定。由于MicroED使用非常小的晶体来确定结构，因此，它比传统的x射线衍射方法有几个优点。在这篇综述文章中，我们讨论了MicroED领域的最新进展及其在不同类型肽、蛋白质、酶、DNA和酶抑制剂络合结构的结构测定中的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Applications of MicroED in structural biology and structure-based drug discovery

查看原文本刊更多论文

Applications of MicroED in structural biology and structure-based drug discovery

Microcrystal electron diffraction (MicroED) is an emerging method for the structure determination of proteins and peptides, enzyme-inhibitor complexes. Several structures of biomolecules, including lysozyme, proteinase K, adenosine receptor A2A, insulin, xylanase, thermolysin, DNA, and Granulovirus occlusion bodies, have been successfully determined through MicroED. As MicroED uses very small crystals for structure determination, therefore, it has several advantages over conventional X-ray diffraction methods. In this review article, we discussed the most recent developments in the field of MicroED and its applications for the structural determination of different types of peptides, proteins, enzymes, DNA, and enzyme-inhibitor-complexed structures.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Biochimica et biophysica acta. General subjects 生物-生化与分子生物学

CiteScore

6.40

自引率

0.00%

发文量

139

审稿时长

30 days

期刊介绍： BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.