{"title":"纳米氧化锌包埋结冷胶海绵促进伤口愈合：理化、抗菌和止血性能的体外研究","authors":"Başak Kurin Atasoyu, Gülşen Bayrak, Selen Öztürk, Aysun Kılıç Süloğlu, Bengi Özkahraman, Işık Perçin","doi":"10.1007/s10924-024-03418-y","DOIUrl":null,"url":null,"abstract":"<div><p>In the current study, a biocompatible zinc oxide (ZnO) nanoparticle embedded gellan gum (GG) (GG-ZnNP) sponge with excellent antibacterial activity, enhanced wound healing and hemostatic properties was presented. Firstly, ZnO nanoparticles were synthesized by co-precipitation methods and characterized through Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis. The size distribution and morphology of the ZnO nanoparticles were investigated by applying dynamic light scattering (DLS) and transmission electron microscopy (TEM). Presence and distribution of the ZnO nanoparticles in GG sponges were examined with Energy-dispersive x-ray spectroscopy (EDX) Thermogravitational Analysis (TGA) conducted to evaluate the thermal stability of GG-ZnO sponges. Following that, ZnO nanoparticles were embedded into GG sponges to enhance their antimicrobial properties. The physicochemical properties of GG-ZnNP sponges were characterized by FTIR, Scanning Electron Microscopy (SEM), swelling and degradation tests. The agar disk diffusion test and colony-forming unit assay findings showed that all GG-ZnNP sponges had a strong antibacterial activity against <i>Escherichia coli (E.coli)</i> and <i>Staphylococcus aureus (S. aureus)</i> bacteria. Furthermore, in vitro blood absorption tests indicated that GG-ZnNP sponges could effectively shorten bleeding time and increase blood absorption capacity. Cell viability studies conducted by MTT and scratch assay with L929 fibroblast cells. MTT assay were performed to found applicable ZnO dose of the sponges to be use as a wound dressings. In vitro scratch assays showed that GG-ZnNP sponges promoted wound closure and re-epithelialization in L929 fibroblast cells at increasing incubation time. GG-ZnNP sponges have a significant and improvable potential for wound dressing applications with their antibacterial and superior hemostatic properties.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>","PeriodicalId":659,"journal":{"name":"Journal of Polymers and the Environment","volume":"33 1","pages":"210 - 229"},"PeriodicalIF":4.7000,"publicationDate":"2024-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ZnO nanoparticle-embedded Gellan Gum Based Sponge for Accelerated Wound Healing: An in vitro Study on Physicochemical, Antibacterial and Hemostatic Properties\",\"authors\":\"Başak Kurin Atasoyu, Gülşen Bayrak, Selen Öztürk, Aysun Kılıç Süloğlu, Bengi Özkahraman, Işık Perçin\",\"doi\":\"10.1007/s10924-024-03418-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>In the current study, a biocompatible zinc oxide (ZnO) nanoparticle embedded gellan gum (GG) (GG-ZnNP) sponge with excellent antibacterial activity, enhanced wound healing and hemostatic properties was presented. Firstly, ZnO nanoparticles were synthesized by co-precipitation methods and characterized through Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis. The size distribution and morphology of the ZnO nanoparticles were investigated by applying dynamic light scattering (DLS) and transmission electron microscopy (TEM). Presence and distribution of the ZnO nanoparticles in GG sponges were examined with Energy-dispersive x-ray spectroscopy (EDX) Thermogravitational Analysis (TGA) conducted to evaluate the thermal stability of GG-ZnO sponges. Following that, ZnO nanoparticles were embedded into GG sponges to enhance their antimicrobial properties. The physicochemical properties of GG-ZnNP sponges were characterized by FTIR, Scanning Electron Microscopy (SEM), swelling and degradation tests. The agar disk diffusion test and colony-forming unit assay findings showed that all GG-ZnNP sponges had a strong antibacterial activity against <i>Escherichia coli (E.coli)</i> and <i>Staphylococcus aureus (S. aureus)</i> bacteria. Furthermore, in vitro blood absorption tests indicated that GG-ZnNP sponges could effectively shorten bleeding time and increase blood absorption capacity. Cell viability studies conducted by MTT and scratch assay with L929 fibroblast cells. MTT assay were performed to found applicable ZnO dose of the sponges to be use as a wound dressings. In vitro scratch assays showed that GG-ZnNP sponges promoted wound closure and re-epithelialization in L929 fibroblast cells at increasing incubation time. GG-ZnNP sponges have a significant and improvable potential for wound dressing applications with their antibacterial and superior hemostatic properties.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><img></picture></div></div></figure></div></div>\",\"PeriodicalId\":659,\"journal\":{\"name\":\"Journal of Polymers and the Environment\",\"volume\":\"33 1\",\"pages\":\"210 - 229\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2024-10-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Polymers and the Environment\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10924-024-03418-y\",\"RegionNum\":3,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Polymers and the Environment","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10924-024-03418-y","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
ZnO nanoparticle-embedded Gellan Gum Based Sponge for Accelerated Wound Healing: An in vitro Study on Physicochemical, Antibacterial and Hemostatic Properties
In the current study, a biocompatible zinc oxide (ZnO) nanoparticle embedded gellan gum (GG) (GG-ZnNP) sponge with excellent antibacterial activity, enhanced wound healing and hemostatic properties was presented. Firstly, ZnO nanoparticles were synthesized by co-precipitation methods and characterized through Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD) analysis. The size distribution and morphology of the ZnO nanoparticles were investigated by applying dynamic light scattering (DLS) and transmission electron microscopy (TEM). Presence and distribution of the ZnO nanoparticles in GG sponges were examined with Energy-dispersive x-ray spectroscopy (EDX) Thermogravitational Analysis (TGA) conducted to evaluate the thermal stability of GG-ZnO sponges. Following that, ZnO nanoparticles were embedded into GG sponges to enhance their antimicrobial properties. The physicochemical properties of GG-ZnNP sponges were characterized by FTIR, Scanning Electron Microscopy (SEM), swelling and degradation tests. The agar disk diffusion test and colony-forming unit assay findings showed that all GG-ZnNP sponges had a strong antibacterial activity against Escherichia coli (E.coli) and Staphylococcus aureus (S. aureus) bacteria. Furthermore, in vitro blood absorption tests indicated that GG-ZnNP sponges could effectively shorten bleeding time and increase blood absorption capacity. Cell viability studies conducted by MTT and scratch assay with L929 fibroblast cells. MTT assay were performed to found applicable ZnO dose of the sponges to be use as a wound dressings. In vitro scratch assays showed that GG-ZnNP sponges promoted wound closure and re-epithelialization in L929 fibroblast cells at increasing incubation time. GG-ZnNP sponges have a significant and improvable potential for wound dressing applications with their antibacterial and superior hemostatic properties.
期刊介绍：
The Journal of Polymers and the Environment fills the need for an international forum in this diverse and rapidly expanding field. The journal serves a crucial role for the publication of information from a wide range of disciplines and is a central outlet for the publication of high-quality peer-reviewed original papers, review articles and short communications. The journal is intentionally interdisciplinary in regard to contributions and covers the following subjects - polymers, environmentally degradable polymers, and degradation pathways: biological, photochemical, oxidative and hydrolytic; new environmental materials: derived by chemical and biosynthetic routes; environmental blends and composites; developments in processing and reactive processing of environmental polymers; characterization of environmental materials: mechanical, physical, thermal, rheological, morphological, and others; recyclable polymers and plastics recycling environmental testing: in-laboratory simulations, outdoor exposures, and standardization of methodologies; environmental fate: end products and intermediates of biodegradation; microbiology and enzymology of polymer biodegradation; solid-waste management and public legislation specific to environmental polymers; and other related topics.
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