Sheng Ma, Sisi Li, Shengyao Jiang, Lirui Wang, Dian Zhan, Manyi Xiong, Yanping Jiang, Qixian Huang, Haozhan Kui, Xinhong Li
{"title":"母体暴露于聚苯乙烯纳米塑料通过诱导未分化精原细胞中Prdm14的过表达来破坏小鼠后代的精子发生","authors":"Sheng Ma, Sisi Li, Shengyao Jiang, Lirui Wang, Dian Zhan, Manyi Xiong, Yanping Jiang, Qixian Huang, Haozhan Kui, Xinhong Li","doi":"10.1021/acsnano.4c10701","DOIUrl":null,"url":null,"abstract":"Undifferentiated spermatogonia (Undiff-SPG) plays a critical role in maintaining continual spermatogenesis. However, the toxic effects and molecular mechanisms of maternal exposure to nanoplastics on offspring Undiff-SPG remain elusive. Here, we utilized a multiomics combined cytomorphological approach to explore the reproductive toxicity and mechanisms of polystyrene nanoplastics (PS-NPs) on offspring Undiff-SPG in mice after maternal exposure. The results indicated that PS-NPs decreased testosterone levels and reduced sperm concentration and quality in offspring male mice through maternal exposure. Moreover, PS-NPs could enter offspring Undiff-SPG, increase ROS levels, and decrease the viability of Undiff-SPG. According to the transcriptomics and proteomics analyses, PS-NPs caused offspring male mice Undiff-SPG inflammation by increasing the expression of <i>Tnfsf18</i>/<i>Nlrp6</i>. Mechanistically, we found that inflammation induced overexpression of the transcription factor <i>Prdm14</i> in Undiff-SPG, which suppressed the expression of <i>Ccdc33</i> and <i>Tcirg1</i>. Additionally, PS-NPs disrupted offspring spermatogenesis by inhibiting the <i>Osbp2</i>/<i>Zcwpw1</i>/<i>Dhps</i> expression. Furthermore, PS-NPs reduced the Undiff-SPG autophagic flux by reducing the expression of <i>Igbp1</i>/<i>Gabarapl2</i>. In conclusion, maternal exposure to PS-NPs caused inflammation in offspring Undiff-SPG, which resulted in <i>Prdm14</i> overexpression that could disrupt spermatogenesis and normal autophagy.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"36 1","pages":""},"PeriodicalIF":15.8000,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Maternal Exposure to Polystyrene Nanoplastics Disrupts Spermatogenesis in Mouse Offspring by Inducing Prdm14 Overexpression in Undifferentiated Spermatogonia\",\"authors\":\"Sheng Ma, Sisi Li, Shengyao Jiang, Lirui Wang, Dian Zhan, Manyi Xiong, Yanping Jiang, Qixian Huang, Haozhan Kui, Xinhong Li\",\"doi\":\"10.1021/acsnano.4c10701\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Undifferentiated spermatogonia (Undiff-SPG) plays a critical role in maintaining continual spermatogenesis. However, the toxic effects and molecular mechanisms of maternal exposure to nanoplastics on offspring Undiff-SPG remain elusive. Here, we utilized a multiomics combined cytomorphological approach to explore the reproductive toxicity and mechanisms of polystyrene nanoplastics (PS-NPs) on offspring Undiff-SPG in mice after maternal exposure. The results indicated that PS-NPs decreased testosterone levels and reduced sperm concentration and quality in offspring male mice through maternal exposure. Moreover, PS-NPs could enter offspring Undiff-SPG, increase ROS levels, and decrease the viability of Undiff-SPG. According to the transcriptomics and proteomics analyses, PS-NPs caused offspring male mice Undiff-SPG inflammation by increasing the expression of <i>Tnfsf18</i>/<i>Nlrp6</i>. Mechanistically, we found that inflammation induced overexpression of the transcription factor <i>Prdm14</i> in Undiff-SPG, which suppressed the expression of <i>Ccdc33</i> and <i>Tcirg1</i>. Additionally, PS-NPs disrupted offspring spermatogenesis by inhibiting the <i>Osbp2</i>/<i>Zcwpw1</i>/<i>Dhps</i> expression. Furthermore, PS-NPs reduced the Undiff-SPG autophagic flux by reducing the expression of <i>Igbp1</i>/<i>Gabarapl2</i>. In conclusion, maternal exposure to PS-NPs caused inflammation in offspring Undiff-SPG, which resulted in <i>Prdm14</i> overexpression that could disrupt spermatogenesis and normal autophagy.\",\"PeriodicalId\":21,\"journal\":{\"name\":\"ACS Nano\",\"volume\":\"36 1\",\"pages\":\"\"},\"PeriodicalIF\":15.8000,\"publicationDate\":\"2025-01-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Nano\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://doi.org/10.1021/acsnano.4c10701\",\"RegionNum\":1,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nano","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsnano.4c10701","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Maternal Exposure to Polystyrene Nanoplastics Disrupts Spermatogenesis in Mouse Offspring by Inducing Prdm14 Overexpression in Undifferentiated Spermatogonia
Undifferentiated spermatogonia (Undiff-SPG) plays a critical role in maintaining continual spermatogenesis. However, the toxic effects and molecular mechanisms of maternal exposure to nanoplastics on offspring Undiff-SPG remain elusive. Here, we utilized a multiomics combined cytomorphological approach to explore the reproductive toxicity and mechanisms of polystyrene nanoplastics (PS-NPs) on offspring Undiff-SPG in mice after maternal exposure. The results indicated that PS-NPs decreased testosterone levels and reduced sperm concentration and quality in offspring male mice through maternal exposure. Moreover, PS-NPs could enter offspring Undiff-SPG, increase ROS levels, and decrease the viability of Undiff-SPG. According to the transcriptomics and proteomics analyses, PS-NPs caused offspring male mice Undiff-SPG inflammation by increasing the expression of Tnfsf18/Nlrp6. Mechanistically, we found that inflammation induced overexpression of the transcription factor Prdm14 in Undiff-SPG, which suppressed the expression of Ccdc33 and Tcirg1. Additionally, PS-NPs disrupted offspring spermatogenesis by inhibiting the Osbp2/Zcwpw1/Dhps expression. Furthermore, PS-NPs reduced the Undiff-SPG autophagic flux by reducing the expression of Igbp1/Gabarapl2. In conclusion, maternal exposure to PS-NPs caused inflammation in offspring Undiff-SPG, which resulted in Prdm14 overexpression that could disrupt spermatogenesis and normal autophagy.
期刊介绍:
ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.