通过反向疫苗学鉴定的Sm050890和Sm141290蛋白表位的免疫保护和诊断潜力评估

IF 1.2 3区 农林科学 Q4 PARASITOLOGY
Flávio Martins de Oliveira, Gabriela Francine Martins Lopes, Rosy Iara Maciel Azambuja Ribeiro, José Augusto Ferreira Perez Villar, Cristina Toscano Fonseca, Débora de Oliveira Lopes
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引用次数: 0

摘要

目的血吸虫病仍然是一种影响全世界数百万人的寄生虫病,需要接种疫苗等干预措施。在之前的工作中,我们的小组使用反向疫苗学从曼氏血吸虫蛋白中鉴定出两个表位,Sm050890(44-58)和Sm141290(225-239)。本研究在小鼠疫苗接种试验中评估了多肽作为免疫原混合物诱导的免疫反应概况和保护作用。此外,这些肽的诊断潜力在免疫分析中进行了评估。方法用含多肽混合物的制剂免疫smice,感染后灌流进行虫负荷回收和定量。采用动物肝脏和血液标本评价免疫对肉芽肿形成和特异性抗肽抗体(IgG)的影响。此外,在免疫小鼠的脾细胞培养中进行细胞因子测量,并使用感染曼氏梭菌个体的外周血血清来评估IgG抗体对肽的识别。结果该疫苗刺激了IgG和IgG2c抗体的产生,与线虫负荷显著降低44 - 29%相关。虽然疫苗没有减轻肝脏病理,但它增加了IFN-γ的产生,同时降低了脾细胞IL-10的产生。此外,感染个体血清中的IgG抗体不能识别Sm050890(44-58)和Sm141290(225-239)肽。总之,我们的数据表明Sm050890(44-58)和Sm141290(225-239)是很有希望的血吸虫病疫苗候选,可以在未来的研究中用于组成多表位/嵌合疫苗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluating the Immunoprotective and Diagnostic Potential of Schistosoma mansoni Epitopes from Sm050890 and Sm141290 Proteins Identified Through Reverse Vaccinology

Purpose

Schistosomiasis remains a parasitic disease affecting millions of people worldwide, requiring interventions like vaccination. In previous work, our group used reverse vaccinology to identify two epitopes from the Schistosoma mansoni proteins, Sm050890 (44–58) and Sm141290 (225–239). This study evaluated the immune response profile and protection induced by peptides, as a mixture of immunogens, in murine vaccination trials. Additionally, the diagnostic potential of these peptides was assessed on immunoassays.

Methods

Mice were immunized with a formulation containing the mixture of the peptides, subsequently infected, and perfused for worm burden recovery and quantification. Liver and blood samples from animals were used to evaluate the effect of immunization on the formation of granulomas and specific anti-peptide antibodies (IgG). Additionally, cytokine measurement was performed in splenocyte cultures from immunized mice, and peripheral blood serum from individuals infected with S. mansoni was used to assess the recognition of the peptides by IgG antibodies.

Results

The vaccine stimulated an increase in the production of IgG and IgG2c antibodies, associated with a significant reduction of 44 − 29% in worm burden. Although the vaccine did not reduce liver pathology, it enhanced the production of IFN-γ while decreasing IL-10 production by splenocytes. Furthermore, the peptides Sm050890 (44–58) and Sm141290 (225–239) were not recognized by IgG antibodies in the serum from infected individuals.

Conclusion

Overall, our data suggest that the peptides Sm050890 (44–58) and Sm141290 (225–239) are promising vaccine candidates against schistosomiasis and can be used to compose a multiepitope/chimeric vaccine in future studies.

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来源期刊
Acta Parasitologica
Acta Parasitologica 医学-寄生虫学
CiteScore
3.10
自引率
6.70%
发文量
149
审稿时长
6-12 weeks
期刊介绍: Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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