Fatemah A.M. Aryan, Petras Prakas, Evelina Juozaitytė-Ngugu, Donatas Šneideris, Zein Abd-Al-Aal, Abd Allah A. Alhoot, Amira I. El-Kabbany, Laila M.A. Tahrani, Osama M.E. El-Azazy
{"title":"科威特市区褐鼠（褐家鼠）中的Cymruensis：详细的形态和分子特征","authors":"Fatemah A.M. Aryan, Petras Prakas, Evelina Juozaitytė-Ngugu, Donatas Šneideris, Zein Abd-Al-Aal, Abd Allah A. Alhoot, Amira I. El-Kabbany, Laila M.A. Tahrani, Osama M.E. El-Azazy","doi":"10.1007/s11686-024-00952-6","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The objective of the study was to establish the prevalence of <i>Sarcocystis</i> (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.</p><h3>Methods</h3><p>Ninety-eight brown rats (<i>Rattus norvegicus</i>) were examined for <i>Sarcocystis</i> spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy. The detected <i>Sarcocystis</i> species was characterised at nuclear <i>18S</i> and <i>28S</i> ribosomal RNA (rRNA), internal transcribed spacers 1 and 2, (<i>ITS1</i> and <i>ITS2</i>), mitochondrial cytochrome c oxidase subunit I (<i>cox1</i>) and cytochrome b (<i>cytb</i>), and apicoplast RNA polymerase beta subunit (<i>rpoB</i>).</p><h3>Results</h3><p>Sarcocysts were found in thigh muscles of 13.3% of the animals examined, while no oocysts/sporocysts were detected in faecal samples. Under a light microscope, sarcocysts were spindle-shaped, 850–3152 × 73–125 μm (1781 ± 763 × 99 ± 15 μm) in size and had thin (up to 0.9 μm) and apparently smooth cyst wall. By transmission electron microscopy, sarcocyst wall was 0.7–1.1 μm in thickness with numerous osmiophilic bleb-like protrusions. Based on DNA sequencing the sarcocysts examined were identified as <i>S</i>. <i>cymruensis</i>. Notably, <i>ITS2</i> and <i>rpoB</i> sequences of <i>S</i>. <i>cymruensis</i> were obtained for the first time. No intraspecific variation was detected comparing <i>28S</i> rRNA, <i>ITS1</i>, <i>cox1</i> and <i>cytb</i> sequences of <i>S</i>. <i>cymruensis</i> isolated from Kuwait, Grenada and China. According to phylogenetic analysis, <i>S</i>. <i>cymruensis</i> was most closely related to <i>S</i>. <i>muris</i>, <i>S</i>. <i>myodes</i> and <i>S</i>. <i>ratti</i> using rodents as their intermediate hosts and cats as their identified or predatory mammals as their presumed definitive hosts.</p><h3>Conclusion</h3><p>We present the first report of <i>S. cymruensis</i> in Kuwait and in Persian Gulf area. The study shed light on the usefulness of different genetic loci for the characterization of <i>Sarcocystis</i> spp. from rodents.</p></div>","PeriodicalId":6932,"journal":{"name":"Acta Parasitologica","volume":"70 1","pages":""},"PeriodicalIF":1.2000,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Sarcocystis Cymruensis in the Brown Rat (Rattus Norvegicus) from an Urban District in Kuwait: Detailed Morphologic and Molecular Characterization\",\"authors\":\"Fatemah A.M. Aryan, Petras Prakas, Evelina Juozaitytė-Ngugu, Donatas Šneideris, Zein Abd-Al-Aal, Abd Allah A. Alhoot, Amira I. El-Kabbany, Laila M.A. Tahrani, Osama M.E. El-Azazy\",\"doi\":\"10.1007/s11686-024-00952-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Purpose</h3><p>The objective of the study was to establish the prevalence of <i>Sarcocystis</i> (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.</p><h3>Methods</h3><p>Ninety-eight brown rats (<i>Rattus norvegicus</i>) were examined for <i>Sarcocystis</i> spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy. The detected <i>Sarcocystis</i> species was characterised at nuclear <i>18S</i> and <i>28S</i> ribosomal RNA (rRNA), internal transcribed spacers 1 and 2, (<i>ITS1</i> and <i>ITS2</i>), mitochondrial cytochrome c oxidase subunit I (<i>cox1</i>) and cytochrome b (<i>cytb</i>), and apicoplast RNA polymerase beta subunit (<i>rpoB</i>).</p><h3>Results</h3><p>Sarcocysts were found in thigh muscles of 13.3% of the animals examined, while no oocysts/sporocysts were detected in faecal samples. Under a light microscope, sarcocysts were spindle-shaped, 850–3152 × 73–125 μm (1781 ± 763 × 99 ± 15 μm) in size and had thin (up to 0.9 μm) and apparently smooth cyst wall. By transmission electron microscopy, sarcocyst wall was 0.7–1.1 μm in thickness with numerous osmiophilic bleb-like protrusions. Based on DNA sequencing the sarcocysts examined were identified as <i>S</i>. <i>cymruensis</i>. Notably, <i>ITS2</i> and <i>rpoB</i> sequences of <i>S</i>. <i>cymruensis</i> were obtained for the first time. No intraspecific variation was detected comparing <i>28S</i> rRNA, <i>ITS1</i>, <i>cox1</i> and <i>cytb</i> sequences of <i>S</i>. <i>cymruensis</i> isolated from Kuwait, Grenada and China. According to phylogenetic analysis, <i>S</i>. <i>cymruensis</i> was most closely related to <i>S</i>. <i>muris</i>, <i>S</i>. <i>myodes</i> and <i>S</i>. <i>ratti</i> using rodents as their intermediate hosts and cats as their identified or predatory mammals as their presumed definitive hosts.</p><h3>Conclusion</h3><p>We present the first report of <i>S. cymruensis</i> in Kuwait and in Persian Gulf area. The study shed light on the usefulness of different genetic loci for the characterization of <i>Sarcocystis</i> spp. from rodents.</p></div>\",\"PeriodicalId\":6932,\"journal\":{\"name\":\"Acta Parasitologica\",\"volume\":\"70 1\",\"pages\":\"\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2025-01-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Parasitologica\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s11686-024-00952-6\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Parasitologica","FirstCategoryId":"3","ListUrlMain":"https://link.springer.com/article/10.1007/s11686-024-00952-6","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Sarcocystis Cymruensis in the Brown Rat (Rattus Norvegicus) from an Urban District in Kuwait: Detailed Morphologic and Molecular Characterization
Purpose
The objective of the study was to establish the prevalence of Sarcocystis (Apicomplexa, Sarcocystidae) in brown rats from Jleeb Al-Shuyoukh, Kuwait, and to describe detected parasites using morphological and DNA analysis methods.
Methods
Ninety-eight brown rats (Rattus norvegicus) were examined for Sarcocystis spp. Obtained sarcocysts were investigated using light microscopy and electron microscopy. The detected Sarcocystis species was characterised at nuclear 18S and 28S ribosomal RNA (rRNA), internal transcribed spacers 1 and 2, (ITS1 and ITS2), mitochondrial cytochrome c oxidase subunit I (cox1) and cytochrome b (cytb), and apicoplast RNA polymerase beta subunit (rpoB).
Results
Sarcocysts were found in thigh muscles of 13.3% of the animals examined, while no oocysts/sporocysts were detected in faecal samples. Under a light microscope, sarcocysts were spindle-shaped, 850–3152 × 73–125 μm (1781 ± 763 × 99 ± 15 μm) in size and had thin (up to 0.9 μm) and apparently smooth cyst wall. By transmission electron microscopy, sarcocyst wall was 0.7–1.1 μm in thickness with numerous osmiophilic bleb-like protrusions. Based on DNA sequencing the sarcocysts examined were identified as S. cymruensis. Notably, ITS2 and rpoB sequences of S. cymruensis were obtained for the first time. No intraspecific variation was detected comparing 28S rRNA, ITS1, cox1 and cytb sequences of S. cymruensis isolated from Kuwait, Grenada and China. According to phylogenetic analysis, S. cymruensis was most closely related to S. muris, S. myodes and S. ratti using rodents as their intermediate hosts and cats as their identified or predatory mammals as their presumed definitive hosts.
Conclusion
We present the first report of S. cymruensis in Kuwait and in Persian Gulf area. The study shed light on the usefulness of different genetic loci for the characterization of Sarcocystis spp. from rodents.
期刊介绍：
Acta Parasitologica is an international journal covering the latest advances in the subject.
Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews.
The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.
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